Final Flashcards
(35 cards)
The central dogma of molecular biology states the information moves from __________ through ______ to ________.
DNA, RNA, Poteins
Name the three types of RNA
A. mRNA
B. tRNA
C. rRNA
_________ occurs in nucleus where DNA acts as a template for _______ production. While _________ occurs in cytoplasm on ribosomes to produce ___________.
Transcription, mRNA. Translation, proteins.
_________ RNA molecules bring amino acids to ribosomes in the cytoplasm
Transfer
During _________ translation is started; during ____________ it continues and during ___________ it stops.
Initiation, elongation, termination
Taking up of DNA from the fluid around the cell is called __________.
Transformation
When male and female bacterial cells are joined by a sex pili, it is called ______________.
Conjugation
Transfer of genes by a phage is called _____________.
Transduction
Enzymes used to cut up DNA are called ____________.
Endonuclease
What does RFLP stand for?
Restriction fragment length polymorphism
The procedure the separates DNA based on size and charge is called ___________________.
Gel electrophoresis
What method can be used in making many copies of a piece of DNA?
PCR (polymerase chain reaction)
What is the F factor?
F factor is a portion of E. coli DNA that carries genes for making sex pili and conjugation
What is an R plasmid?
Plasmid that carries genes for antibiotic resistance
Give an example of where nucleic acid probes are used.
Testing if a person
carries the genes for cystic fibrosis
What are the advantages of using bacteria or yeast for production of human
proteins?
They grow fast. Easy and cheap to grow them
Where is reverse transcriptase used?
Making DNA from mRNA
What does DNA sequencing accomplish?
Find ATCG sequences of an organism
Use of farm animals to produce human proteins is called ___________.
Pharming
What are plasmids?
Extra-chromosomal pieces of DNA found in bacteria and
yeast
What is the name of the product when a foreign DNA has been inserted into a
plasmid?
Recombinant DNA
Name one product produced by recombinant DNA technology.
Insulin
What enzyme is used to attach pieces of DNA together?
DNA ligase
Name one use of DNA fingerprinting.
Paternity testing to find out who is the
father of a baby