Glycolysis

Question 1

Investment phase

Answer 1

1. Hexokinase rearranges glucose to G6P
2. Glucose 6-phosphate is rearranged to form isomer fructose 6-phosphate. Input of ATP needed
3. Using ATP and another kinase, a second phosphate is added
4. Phosphofructokinase splits fructose-1,6-bisphosphate in half, giving 2x 3C molecules with a phosphate attached at one end.

Question 2

Energy Pay off phase

Answer 2

4. 3C molecule is phosphorylated (input of 2 NAD+) to form 2x3 C molecules with phosphate on both ends and 2 NADH+ H+
5. Product is dephosphorylated where P is used to convert 2x ADP to 2x ATP and 3C molecules with one phosphate on the end.
6. 3C molecules dephosphorylated again to convert 2x ADP to 2x ATP and 2x pyruvate molecules.

Question 3

Total yield

Answer 3

2x ATP, 2x Pyruvate, 2x NADH

Question 4

Glucose O2

Answer 4

All tissues, wholly cytosolic, anaerobic, fast, inefficient, ATP generation almost irrelevant