Hema 2 Lab M2

Question 1

Small, round, or oval, anucleate cytoplasmic fragments of megakaryocytes

Answer 1

Platelets (Thrombocytes)

Question 2

Most accurate way of platelet count

Answer 2

Reese-Ecker

Question 3

True blood cells and produced in the bone marrrow

Answer 3

Platelets (Thrombocytes)

Question 4

Maturation period of Thrombocytes

Answer 4

5 Days

Question 5

Lifespan of Thrombocytes

Answer 5

8-11 days

Question 6

Anticoagulant used in Platelet count

Answer 6

EDTA

Question 7

Immediate precursor of Thrombocytes

Answer 7

Megakaryocytes

Question 8

Precursor of Megakaryoctes

Answer 8

Megakaryoblasts

Question 9

Functions of the Thrombocytes

Answer 9

Prevents bleeding
Maintains homeostasis
Wound healing

Question 10

What are the factors released by the thrombocytes?

Answer 10

Fibrinogen
Thrombin
Prothrombin

Question 11

Also known as thrombocytes

Answer 11

Platelets

Question 12

Recommended method for manual platelet count

Answer 12

Brecher-Conkrite method

Question 13

Purpose of the Reese-Ecker fluid

Answer 13

Preserve and stain the thrombocytes

Question 14

Type of pipette used in the Reese-Ecker method

Answer 14

RBC pipette

Question 15

How many large central squares are counted in the Reese-Ecker method?

Answer 15

25 large central squares

Question 16

Reference range of platelet count

Answer 16

150,000-450,000/ uL
150- 450 x 109/ L

Question 17

Refers to the adherence of platelets around neutrophils, producing a ring or satellite effect and occurs when an EDTA anticoagulant is used

Answer 17

Platelet satellitism

Question 18

Decreased number of circulating platelets

Answer 18

Thrombocytopenia

Question 19

Abnormally high platelet count, typically 450,000/uL

Answer 19

Thrombocytosis

Question 20

What is the color of thrombocytes?

Answer 20

Gray-blue with purple granules

Question 21

Reagents in Guy and Leake’s Method

Answer 21

40% formaldehyde (HCHO), sodium citrate, crystal violet

Question 22

TRUE or FALSE
Platelets must be counted within 2 hours if venous blood is used.

Answer 22

FALSE

dapat 3 hours

Question 23

Advantages of Automated platelet counting

Answer 23

1. Speed of performance
2. Elimination of visual fatigue of the technician
3. Improved precision
4. Reduction in pre-analytic and post-analytic variables
5. Corrects identification of sample for processing and analysis, storage, and retrieval

Question 24

graphic representation of a collection of data based on cell size and/or cell number decipting variations in the process.

Answer 24

Histogram

Question 25

Histogram is also called ______

Answer 25

frequency of distribution curve

Question 26

x axis

Answer 26

cell volume cell

Question 27

y axis

Answer 27

cell number

Question 28

platelets with a size of 8-12 fL are counted between ______

Answer 28

2 and 25 fL

Question 29

Can be counted as platelets, hence an increase in PLT and decrease in RBC

Answer 29

Microcytes & schistocytes

Question 30

Ideal microscope used for platelet count

Answer 30

Phase contrast microscope

Question 31

Ratio of platelets : RBC

Answer 31

1:20

Question 32

What is the platelet value of thrombocytopenia?

Answer 32

lower than 100,000/uL

Question 33

Refers to the adherence of platelets around neutrophils, producing a ring or satellite effect

Answer 33

Platelet satellitism

Question 34

What anticoagulant will use to correct platelet satellitism?

Answer 34

sodium citrate

Question 35

TRUE or FALSE Skin puncture specimen is less desirable because of the tendency of the platelets to aggregate or form clumps.

Answer 35

TRUE

Question 36

100,000 - 149,000/ul

Answer 36

Slight decrease

Question 37

Formula for Platelet Estimate

Answer 37

Formula: 𝑷𝒍𝒂𝒕𝒆𝒍𝒆𝒕𝒔/𝒖𝑳 = 𝑵𝒐. 𝒐𝒇 𝑷𝒍𝒂𝒕𝒆𝒍𝒆𝒕𝒔 𝑪𝒐𝒖𝒏𝒕𝒆𝒅 𝒙 𝟐𝟎, 𝟎𝟎𝟎

Question 38

In the modified Indirect Method, the smear will be stained by ________.

Answer 38

Wright’s or Giemsa stain

Question 39

What type of Indirect method uses 14% Mg as a reagent?

Answer 39

Fonio’s Method

Question 40

In the modified indirect method, how many fields will we count?

Answer 40

10 consecutive OIO fields

Question 41

How many drops should we expel after shaking/distribution of specimen and diluting fluid.

Answer 41

4-5 drops

Question 42

Diagnose bleeding disorders and monitor conditions affecting BM

Answer 42

Platelet count

Question 43

Estimate of platelets per field

Answer 43

8-20