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mls 3H > Hema 2 Lab M2 > Flashcards

Hema 2 Lab M2 Flashcards

1
Q

Small, round, or oval, anucleate cytoplasmic fragments of megakaryocytes

A

Platelets (Thrombocytes)

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2
Q

Most accurate way of platelet count

A

Reese-Ecker

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3
Q

True blood cells and produced in the bone marrrow

A

Platelets (Thrombocytes)

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4
Q

Maturation period of Thrombocytes

A

5 Days

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5
Q

Lifespan of Thrombocytes

A

8-11 days

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6
Q

Anticoagulant used in Platelet count

A

EDTA

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7
Q

Immediate precursor of Thrombocytes

A

Megakaryocytes

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8
Q

Precursor of Megakaryoctes

A

Megakaryoblasts

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9
Q

Functions of the Thrombocytes

A

Prevents bleeding
Maintains homeostasis
Wound healing

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10
Q

What are the factors released by the thrombocytes?

A

Fibrinogen
Thrombin
Prothrombin

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11
Q

Also known as thrombocytes

A

Platelets

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12
Q

Recommended method for manual platelet count

A

Brecher-Conkrite method

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13
Q

Purpose of the Reese-Ecker fluid

A

Preserve and stain the thrombocytes

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14
Q

Type of pipette used in the Reese-Ecker method

A

RBC pipette

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15
Q

How many large central squares are counted in the Reese-Ecker method?

A

25 large central squares

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16
Q

Reference range of platelet count

A

150,000-450,000/ uL
150- 450 x 109/ L

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17
Q

Refers to the adherence of platelets around neutrophils, producing a ring or satellite effect and occurs when an EDTA anticoagulant is used

A

Platelet satellitism

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18
Q

Decreased number of circulating platelets

A

Thrombocytopenia

19
Q

Abnormally high platelet count, typically 450,000/uL

A

Thrombocytosis

20
Q

What is the color of thrombocytes?

A

Gray-blue with purple granules

21
Q

Reagents in Guy and Leake’s Method

A

40% formaldehyde (HCHO), sodium citrate, crystal violet

22
Q

TRUE or FALSE
Platelets must be counted within 2 hours if venous blood is used.

A

FALSE

dapat 3 hours

23
Q

Advantages of Automated platelet counting

A
  1. Speed of performance
  2. Elimination of visual fatigue of the technician
  3. Improved precision
  4. Reduction in pre-analytic and post-analytic variables
  5. Corrects identification of sample for processing and analysis, storage, and retrieval
24
Q

graphic representation of a collection of data based on cell size and/or cell number decipting variations in the process.

A

Histogram

25
Q

Histogram is also called ______

A

frequency of distribution curve

26
Q

x axis

A

cell volume cell

27
Q

y axis

A

cell number

28
Q

platelets with a size of 8-12 fL are counted between ______

A

2 and 25 fL

29
Q

Can be counted as platelets, hence an increase in PLT and decrease in RBC

A

Microcytes & schistocytes

30
Q

Ideal microscope used for platelet count

A

Phase contrast microscope

31
Q

Ratio of platelets : RBC

A

1:20

32
Q

What is the platelet value of thrombocytopenia?

A

lower than 100,000/uL

33
Q

Refers to the adherence of platelets around neutrophils,
producing a ring or satellite effect

A

Platelet satellitism

34
Q

What anticoagulant will use to correct platelet satellitism?

A

sodium citrate

35
Q

TRUE or FALSE
Skin puncture specimen is less desirable because of the
tendency of the platelets to aggregate or form clumps.

A

TRUE

36
Q

100,000 - 149,000/ul

A

Slight decrease

37
Q

Formula for Platelet Estimate

A

Formula:
𝑷𝒍𝒂𝒕𝒆𝒍𝒆𝒕𝒔/𝒖𝑳 = 𝑡𝒐. 𝒐𝒇 𝑷𝒍𝒂𝒕𝒆𝒍𝒆𝒕𝒔 π‘ͺ𝒐𝒖𝒏𝒕𝒆𝒅 𝒙 𝟐𝟎, 𝟎𝟎𝟎

38
Q

In the modified Indirect Method, the smear will be stained by ________.

A

Wright’s or Giemsa stain

39
Q

What type of Indirect method uses 14% Mg as a reagent?

A

Fonio’s Method

40
Q

In the modified indirect method, how many fields will we count?

A

10 consecutive
OIO fields

41
Q

How many drops should we expel after shaking/distribution of specimen and diluting fluid.

A

4-5 drops

42
Q

Diagnose bleeding disorders and monitor conditions affecting BM

A

Platelet count

43
Q

Estimate of platelets per field

A

8-20

mls 3H (12 decks)

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