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Flashcards in Immuno Q Bank Deck (75)
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1

under what conditions do T cells become anergic?

when it interacts with APC in absence of co-stimulatory interactions (CD28/B7)

2

which CDs do double positive thymocytes contain?

CD2, CD3, CD4 and CD8

3

somatic recombination occurs for which TCR chain first? where in the body is the T cell when this occurs?

TCRB

it occurs in the thymus (cortex)

4

what factor activates NK cells? what cell secretes this factor? what do activated NK cells secrete and what is its role?

IL-12 activates NK cells. Dendritic cells (mostly) secrete this. 

activated NK cells secrete IFNγ, which drives subset to form TH1 cells

5

what complements make up classical pathway C5 convertase? Alternative convertase?

classical C5 convertase: C4b2a3b

alternative C5 convertase: C3b3bBb

6

what are the constant regions associated with light chains?

kappa and lambda

7

what heavy chain constant regions exist? what antibodies do they form?

delta = IgD

mu = IgM

8

how many CD79a/b heterodimers are associated with a single mIg?

2

9

which factor initiates clonal expansion of naive CD4+ T cells? What is it secreted by and when? Where does it bind? What is this type of interaction called?

 

IL-2 initiates clonal expansion of naive CD4+ T cells. IL-2 is secreted by naive CD4+ T cells, after it is bound to antigen+APC. 

IL-2 binds to the receptor on itself.

This is called cognate interaction.

10

what does C1 bind to? which results in better activation? why?

C1 binds to the Fc region of IgG and IgM

IgM leads to better activation because it is a pentamer

11

expression of what cytokines and adhesion molecules does macrophage secretion of IL-1 and TNFa induce?

VCAM-1, ICAM-1, MCP-1 (CCL-2), IL-8, E-selectin

12

what cells have FcγRI?

phagocytes; IgG binds to microbe, which then binds to FcγRI which signals the cell to perform phagocytosis

13

what cell is the most efficient APC?

dendritic cell

14

in which stage is CD20 expressed? CD19? CD40?

CD20 = Pre-B cell

CD19 = Pro-B cell

CD40 = naive/mature B-cells

15

which 2 cells are fused to form hybridoma?

plasma cell and myeloma cell

16

which antibody isotype has the highest serum concentration?

IgG

17

what is another name for CD154? CD152?

CD154 = CD40L

CD152 = CTLA4

18

what type of isotypes can be generated following switch recombination?

IgE, IgG, and IgA

19

which antibody exists only in membrane bound form?

IgD

20

what complement receptors do mast cells and basophils have?

CR3a/4a, CR5a

21

which is the only antibody isotype not secreted by plasma cells?

IgD

22

where in the body are B-cells located during VDJ recombination?

bone marrow

23

which antibody isotypes are associated with J chain?

IgA (dimer) and IgM (pentamer)

24

which isotype can cross the placenta?

IgG

25

what induces development of Th1 subset? Th2?

Th1 = IFNγ

Th2 = IL-4

26

which complement causes chemotaxis? of which cells?

C5a = neutrophils

27

what do Th2 cells secrete?

IL-4, IL-5, IL-6, IL-13 and TGF-B

28

which antibody is also known as reaginic antibody?

IgE (reaginic = allergic activity)

29

what does CD28 bind to? on what cell is CD28 located? what is another CD that binds to B7?

CD28 binds to B7 (CD80/86)

CD28 is located on the T cell

another CD that binds to B7 is CTLA-4 (CD152), which is inhibitory

30

what is the role of Properidin?

stabilizes C3bBb (C3 convertase)