Microbiome and Biology of Bacteria Flashcards Preview

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Flashcards in Microbiome and Biology of Bacteria Deck (10)
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1

name the normally sterile sites where microorganisms are not found

  • blood
  • CSF
  • pleural fluid
  • peritoneal & pericardial fluid
  • bone
  • joint fluid
  • internal body sites 
    • brain, heart, lymph, etc.

2

describe the mechanisms by which microbiota provide colonization inhibition/resistance

  • compete for space: occupation of specific receptor blocks access by others
  • produce inhibiting or damaging substances
    • e.g. bacteriocins, lower local pH, etc.
  • compete more effectively for resources
    • limit availability to pathogens
  • stimulate host production of immunomodulatory molecules and anti-inflammatory components 

3

describe unique genetic features of prokaryotes

  • basic genome: double stranded DNA: single chromosome
  • organization: circular or linear (less common)
  • associated proteins: no
  • ribosomes: small, 70s
  • location: nucleoid
  • gene structure: haploid

4

describe the process of bacteria replication (binary fission)

  • replication of chromosome
  • proportional increase in cellular contents
  • elongation
  • separation of chromosome
  • invagination of membrane and cell wall
  • septum formation

5

describe the calculation of generation time

g = mean time elapsed divided by number of generations

example: 210 minutes / 10 generations = g = 21 min.

  • fast generation times can result in short incubation times before onset of disease
  • slow generation times increase time needed for culture 

6

which term best describes the energy and nutritional requirements plus growht environment necessary for pathogenic bacteria, fungi, protozoa and humans?

chemoorganotroph

7

describe the energy generation pathways for bacteria that undergo aerobic respiration vs anaerobic respiration vs fermentation

8

describe how a lab determines what is in a clinical specimen

9

describe the types of bacteriologic media used for clinical microbiology

10

describe the purpose of mannitol salt agar