Intestinal Parasite I (PART 2) Flashcards Preview

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Flashcards in Intestinal Parasite I (PART 2) Deck (30):
1

Enteric amoeba that lyses tissue

Entamoeba histolytica

2

Entamoeba histolytica:
- diseases

1. Amoebic dysentery
2. Amoebic colitis
3. Amoebic abscesses

3

Entamoeba histolytica:
- Geographic distribution

world wide but higher incidence of infection in developing countries

4

Entamoeba histolytica:
- who are the hosts

humans are the only hosts

5

Entamoeba histolytica:
- infectious form

cyst

6

Entamoeba histolytica:
- how are cysts transmitted

fecal-oral rout

7

Entamoeba histolytica:
- test of choice for diagnosis

EIA - high sensitivity and specificity

8

Entamoeba histolytica:
- must be distinguished from ______ _______ and other protozoa and also must be distinguished from ________ ______!!!

- nonpathogenic amoebae
- polymorphonuclear neutrophils

9

Entamoeba histolytica:
- is morphologically indistinguishable from what two organisms

Entamoeba dispar
Entamoeba moshkovskii

10

How do you differentiate Entamoeba histolytica from Entamoeba hartmanni?

Exactly the same just much smaller

11

How do you differentiate Entamoeba histolytica trophs from Entamoeba coli trophs?

E. histolytica: Karyosome central and compact, perifpheral chromatin smoothly and evenly arranged "clean" cytoplasm
E. coli: Karyosome: off-center (messy looking), spread out, peripheral chromatin clumpy and unevenly arranged "dirty" cytoplasm

12

How do you differentiate Entamoeba histolytica cysts from Entamoeba coli cysts?

E. histolytica: Nuclei usually paired on each side in 2 different focal planes, chromatic bodies with rounded ends
E. coli: Nuclei scattered randomly throughout cyst in many different focal planes, chromatoid bodies with splintered ends

13

Only known pathogenic ciliate of humans

Balantidium coli

14

Balantidium coli:
- primary reservoirs

PIGS, rodents, and primates (infections are more common in areas where pigs are raised)

15

Balantidium coli:
- infectious form

cyst

16

Balantidium coli:
- What do we detect in the stool?

trophs seen, cysts not usually seen

17

Balantidium coli:
- appearance of trophs

CILIATED Big (40-200micrometers) cell with bean-shaped macronucleus, cytosome at narrower end

18

Balantidium coli:
- appearance of cyst

CILIATED, very large and round large kidney bean shaped macronucleus

19

What is one of the most common intestinal parasites in the world?

Giardia intestinalis

20

Giardia intestinalis:
- other names

G. lamblia
G. duodenalis

21

Giardia intestinalis:
- Geographic distribution

worldwide; more prevalent in warm climates and in children (daycare)

22

Giardia intestinalis:
- How is it transmitted?

fecal-oral rout, food, person to person
- FOMITES: inanimate objects
- WATERBORNE: most big outbreaks

23

Giardia intestinalis:
- common settings to see this kind of outbreak

- children in daycares
- campers and backpackers (drinking contaminated water)

24

Giardia intestinalis:
- infectious form
- what form comes out?

In: cyst
Out: cyst and troph

25

Giardia intestinalis:
- symptoms

diarrhea, bloating, nausea, malaise, foul-smelling feces and flatulence (purple burps)

26

Giardia intestinalis:
- Test of choice

EIA - high sensitivity and specificity

27

Giardia intestinalis:
- how often are the parasites in the feces?

varies from day to day (get 3 samples over period of 10 days)

28

Giardia intestinalis:
- what motility should you look for in a fresh specimen?

"falling leaf" motility

29

Giardia intestinalis:
- troph appearance

Troph: external flagella, pear shape; two nuclei (look like eyes) sucking disc, median bodies,

30

Giardia intestinalis:
- troops appearance

cyst: small oval cyst, two to 4 nuclei at one end, median bodies across middle, fibrils down center