Intestinal Parasite II (PART 2)

Question 1

What are opportunistic, obligate intracellular pathogens?

Answer 1

Microsporidia

Question 2

Microporidia:

- not protozoa…what are they?

Answer 2

fungi

Question 3

Two types of microsporidia that are common human gastrointestinal pathogens

Answer 3

Enterocytozoon bieneus

Encephalitozoon intestinalis

Question 4

Microporidia:

- Who are most of its victims?

Answer 4

severely immunocompromised AIDS patients represent most victims

Question 5

Microporidia:

- infectious form

Answer 5

spore

Question 6

Microporidia:

- What stain should you use for microscopic exam

Answer 6

Chromotrope 2R and Ryan’s modified-trichrome stain

Question 7

Microporidia:

• Gold standard test
• test of choice

Answer 7

Gold: Transmission electron microscopy
Choice: immunofluorescence

Question 8

Encephalitozoon species

- appearance

Answer 8

in gram stain, resembles large GPRs

Question 9

Blastocystis species:

- Infectious form

Answer 9

cyst (cyst released also)

Question 10

Blastocystis species:

- role in disease

Answer 10

debateable role (sometimes no symptoms other times diarrhea etc.)

Question 11

Chilomastix mesnili:

- pathogenic or nonpathogenic

Answer 11

nonpathogenic protozoa

Question 12

Chilomastix mesnili:

- appearance of trophs

Answer 12

External flagella, lopsided shortened pear shape, one nucleus, cytosome looks like mouth (smiling fish)

Question 13

Chilomastix mesnili:

- appearance of cysts

Answer 13

small lemon shape cyst, one nucleus, cytosine small, looks like a shephard’s crook

Question 14

Endolimax nana:

- pathogenic or nonpathogenic

Answer 14

nonpathogenic

Question 15

Endolimax nana:

- appearance of troph

Answer 15

without peripheral nuclear chromatin, large irregular karyosome (nana with dilated pupil)

Question 16

Endolimax nana:

- appearance of cyst

Answer 16

four nuclei (nana has 4 eyes), short oval shape, small nuclei surrounded by clear space

Question 17

Enteromonas hominis:

- path or non path

Answer 17

nonpath

Question 18

Enteromonas hominis:

- appearance

Answer 18

?

Question 19

Iodamoeba butschlii:

- path or non path

Answer 19

nonpath

Question 20

Iodamoeba butschlii:

• cyst appearance
• troph appearance

Answer 20

cyst: large vacuole in the cyst

Question 21

Pentatrichomonas hominis:

- path or non path

Answer 21

nonpath

Question 22

Pentatrichomonas hominis:

- appearance of trophs

Answer 22

external flagella, lopsided pear shape, one nucleus, undulating membrane and axostyle (fish with spike)