Intro to Virology Flashcards
What type of viral classification is Hep B a part of?
Family:Hepadnaviridae, DNA Nucleic Core, DS part circular nucleic acid, enveloped virion, spherical capsid symmetry
What type of viral classification is smallpox a part of?
Family: Poxviridae, DNA nucleic core, DS linear nucleic acid, Enveloped virion, Brick-shaped capsid symmetry
What type of viral classification is HIV a part of?
RNA nucleic core, Icosahedral eveloped capsid symmetry, Diploid single strand positive, Retroviridae family
Observed virus-induced cytopathologic effects on cells
Cowdry type A nuclear inclusion bodies, Negri bodies, Nuclear owl’s eye inclusions, cell lysis, syncytia
What is the gold standard for viral Dx?
Isolate and culture the etiologic agent or PCR
Tissue culture dose 50
Titer of virus causing CPE in 50% of cells
Lethal dose 50
titer of virus killing 50% of test animals
Infectious dose 50
titer of virus infecting 50% of test animals
Plaque-forming units
CPE units used to observe a viral colony
Seroconversion
4-fold increase in titer between acute and convalescents phases that proves identity of disease agent
Type IV hypersensitivity
Allergic rxn that does NOT involve Ab. Sensitized T-lymph react directly with viral Ag, producing lysis
Tzanck test
Syncytia produced by viral infections. Used to Dx HSV
Cell Transformation from virus
Integration of viral nucleic acid into the host chromosome. Commonly causes cancer
Type II hypersensitivity
IgG/M. viral Ab fixed to cell, causing lysis. Anemia is a common clinical manefestation
Pyknosis
Nucleus pushed to eccentric position in the cell (rabies)
