Introduction to Bacteria (Exam 1) Flashcards

(54 cards)

1
Q

What diseases are commonly associated with AIDS

A

Cryptococcus neoformans
Cryptosporidium parvum
Pneumocystis jiroveci

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2
Q

What organism is often associated with cystic fibrosis

A

pseudomonas

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3
Q

What organism is often associated with sickle cell anemia

A

Salmonella

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4
Q

What are the gram positive cocci bacteria

A
staph aureus
staph saprophyticus
staph pyrogenes
staph argalactiae
staph pneumonia
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5
Q

What are the gram positive rod bacteria

A

bacillus anthracis
clostridium botulinum
corneybacterium diptheriae
listeria monocytogenes

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6
Q

What are the gram negative rods

A

Escherichia coli
Salmonella typhi
Vibrio cholerae
Haemophillus ducreryi

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7
Q

What are the gram negative cocci

A

neisseria gonorrhea
neisseria menigitidis
maraxella catarrhalis

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8
Q

What are the spiral gram negative bacteria

A

borrellia buurgdoferi
leptospira interrogams
treponema pallidum

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9
Q

What type of bacteria will form spores

A

Bacillus

Clostridium

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10
Q

How will mycoplasma appear on a gram stain and why

A

will not appear due to lack of cell wall

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11
Q

What will be seen in place of seeing mycoplasma on a gram stain

A

neutrophils showing sign of infection

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12
Q

What are examples of direct examination methods

A

wet mount
KOH prep
contrasting dyes

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13
Q

What is a wet mount used for

A

parasites and fungi

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14
Q

What is KOH prep used for

A

dissolve background material

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15
Q

What are the contrasting dyes

A

lactophenol cotton blue, iodine, india ink

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16
Q

What are examples of differential stains

A

gram staining
Silver stain
Wright-Giemsa stain
Acid fast stains

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17
Q

What is Silver stain used for

A

H. pylori

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18
Q

What is Wright Giemsa stain used for

A

blood parasites and inclusion bodies

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19
Q

What is acid fast stains used for

A

TB

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20
Q

What is the only gram positive diplococci

A

strep pneumo

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21
Q

How will chlamydia appear on gram stain and why

A

just see neutrophils, no bacteria. It is an intracellular bacteria

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22
Q

What is an obligate anaerobe

23
Q

What is an example of an obligate anaerobe bacteria

24
Q

What is the appearance of anthrax on gram stain

A

box-car like arrangement

gram positive rods with spores

25
What are pleomorphic rods
resemble a tennis racquet or tadpole
26
What are examples of curved gram negative rods
vibrio cholera | all GI bugs
27
What are bipolar rods
resemble a safety pin, stained only on the ends
28
WHat type of organisms can be seen on wet mount
parasites and fungi
29
What type of organisms will appear on iodine stain
parasites
30
What is an india ink stain used to view
capsule
31
What stains can be used to view fecal protozoa
iron hematoxylin | trichrome stain
32
What organisms can be seen on methenamine silver stain
parasites, PCP
33
What organisms can be viewed on calcofluor white stain
fungi
34
What is the use of inert labe media
used to facultative intracellular or excellular bacteria
35
What characteristics can inert labe media be
non-selective selective/differential specialized
36
How are obligate intracellular organisms cultured
unable to culture these organisms
37
What are the different hemolysis patterns seen on blood agar
beta alpha gamma
38
What is beta hemolysis
complete hemolysis: clear
39
What is alpha hemolysis
incomplete lysis: green pigment
40
What is gamma hemolysis
no hemolysis; just grows
41
What organisms that grow on blood agar grow on chocolate agar
all of them
42
What is MacConkey agar selective and differential for
selective: gram negative rods differential: lactose fermenters
43
What does osin methylene blue agar differentiate
lactose fermenters
44
What is Hektoen Enteric Agar used to differentiate
Salmonella
45
What does salmonella produce on a Hektoen enteric agar plate
black pigment (H2S)
46
What agar is differential for EHEC
sorbital MacConkey (SMAC) agar
47
What is bile esculin agar differential for
enterococcus
48
What is mannitol salt agar differential for
staph aureus
49
What agar is used to culture legionella
buffered charcoal yeast extract agar
50
What agar is used to culture vibrio
thiosulfate citrate bile salts sucrose agar
51
What organism can be cultured on sabouraud dextrose agar
fungi
52
What are the miscellaneous growth requirements of mycoplasma
cholesterol and pruines | pyrimidines
53
What are the miscellaneous growth requirements of Francisella tulatensis
cysteine and iron
54
What are the miscellaneous growth requirements of Haemophilus influenza
X (heme) and V (NAD) factors