L16- 18: Cloning and Hydribisation Flashcards Preview

SCIE1106 > L16- 18: Cloning and Hydribisation > Flashcards

Flashcards in L16- 18: Cloning and Hydribisation Deck (13)
Loading flashcards...
1

define recombinant dna

molecule of dna that has originated from 2 or more dna fragments

2

define cloning

production of identical copies of partiliar dna molecule

3

what do restriction enzyme sdo

cut dna at specific sites
leaving 3' hydroxyl and 5' phosphate groups
-can be blunt or sticky ends

4

function of restriction enzymes

protect cells from invaders
act together with methylase

5

what does dna ligase do to annealed dna

fill in cap of sugar phosphate bonds
covalently link

6

what is a plasmid

circular dna molecule

7

how do you make bacterial cells permeable to dna

heat shock or electroporation

8

process of electrophoresis

dna carries negative charge and migrates to pos electrodes

9

why are plasmid vectors used in cloning

-moved from cell to cell easily.
- contain the genes that control their own replication.
- no detrimental effects on their host cells.

10

what does hydrbidiation occur between

2 dna
2 RNA > double stranded rna
dna + rna= hybrid

11

3 techniques for nucleic acid hybridisation

southern/northern blotting
in situ hybridisation

12

3 steps of PCR

denaturing
annealing
extending

13

explain pcr

- temp increase= unwinds + separated
temp decrease= primers attach to complementary
primers annealed + taq polymerase copies dna from primer and adds nucleotides
temp increas= strand seperated