Flashcards in L66 Deck (15):
1
What are the 8 steps in viral replication?
1. Recognition
2. Attachment
3. Penetration/entry
4. Uncoating
5. Macromolecule synthesis
6. Assembly
7. Budding - if enveloped virus
8. Release
2
What is a VAP?
Viral attachment protein
- Subject to hypermutation (poses Ab immunity challenge)
- Binds cellular receptor --> viral recognition & attachment to host cell
3
Is recognition/attachment E dependent? Entry?
R&A - passive
Entry - E dep, requires metabolically active cell
4
What are the 3 patterns of viral entry into host cell?
1. Envelope fuses @ plasma membrane - immediate release of capsid+genetic material
2. Enveloped virus taken up as endosome - fusion in endosome to release capsid+genetic
3. Naked virus in endosome & released by decreased pH
5
What is the order of macromolecule synthesis?
1. Early mRNA + regulatory proteins
2. Genome replication - cytoplasm or nucleus
3. Late mRNA + structural proteins
4. Modify proteins made
6
What is the eclipse period of viral growth?
Once virus has entered host cell but disassembled - no virions
7
What is the latent period of viral growth?
Between viral infection --> detection as extracellular virus (aka all intracell time)
8
What type of viruses have a detectable intracellular period of viral growth?
Viruses released by cell lysis
9
What is burst size?
Ratio
Infected virus particles released : cell number
10
What is the cytopathic effect?
Changes to host cell due to viral infection
Ex:
- Herpes - syncytia
- Flu - RBCs together
11
What is the genome of adenovirus? Encapsulated?
dsDNA
Naked icosahedral
12
What diseases does adenovirus case?
Pink eye
RTIs, diarrhea, hemorrhagic cystitis
13
What is the immune response to adenovirus?
Abs
14
What is the genome of polyviruses?
dsDNA
Replicates @ cytoplasm
15