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Bacteriology > Lab 6 Salmonella, Citrobacter, Shigella, Proteus, Providentia, Yersinia, Serratia, Edwardsiella, anaerobic Gram-negative rods > Flashcards

Lab 6 Salmonella, Citrobacter, Shigella, Proteus, Providentia, Yersinia, Serratia, Edwardsiella, anaerobic Gram-negative rods Flashcards

1
Q

Salmonella species

A

Only two:
S. Enterica
S. Bongori

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2
Q

Within s. Enterica

A

6 subspecies

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3
Q

Within subspecies

A

Serovars

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4
Q

Salmonella habitat

A

Gut
Sewage
Water
Feed

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5
Q

Salmonella morphology

A

Rod, flagella

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6
Q

What salmonella do not have flagella

A

S. Gallinarum

S. Pullorum

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7
Q

Salmonella culture

A

Not fastidious
Low selectivity: MacConkey, Drigalski
Medium selectivity: brill green phenol red, XLD, rambach agar

Selective enrichment
Na-selenite, cys
Na-tetrathionate, bile salts

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8
Q

Salmonella is lactose

A

Negative

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9
Q

Salmonella on rambach agar

A

Pink colonies

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10
Q

Salmonella H2S

A

Positive

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11
Q

Salmonella antigens

A

Kauffmann-white system
O = 35
Vi = K, S.typhi, s. Dublin
H = specific, non-specific, monophasic, diphasic

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12
Q

Salmonella only human

A

S. Typhi

S. Paratyphi A B C, S. Sendai

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13
Q

S. Typhi

A

Human typhoid fever

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14
Q

S. Paratyphi, S. Sendsai

A

Human paratyphoid

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15
Q

Salmonella only animals

A

S. Typhisuis

S. Gallinarum

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16
Q

S. Typhisuis

A

Swine typhoid

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17
Q

S. Gallinarum (pullorum)

A

Fowl paratyphoid

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18
Q

Salmonella human and animal

A

S. Enteritidis
S. Typhimurium
S. Thompson
S. Choleraesuis

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19
Q

Citrobacter habitat

A

Mammals, birds, gut, water, environment

20
Q

Cireobacter lactose

A

Negative

Late positive

21
Q

Citrobacter pathogenicity

A

Saprophytic

Sometimes secondary pathogen

22
Q

Shigella habitat

A

Intestine of primates, sewage

23
Q

Shigella flagella

A

No flagella

24
Q

Shigella culture

A

DC agar

25
Q

Shigella lactose

A

Negative

26
Q 
Shigella
S. Dysenteriae
S.flexneri
S. Sonnei
S. Boydii
A

Human bacillary dysentery

27
Q

Shigella mannitol and H2S

A

Negative both

28
Q

Proteus/providencia habitat

A

Intestine
Urine
Water
Rotting materials

29
Q

Proteus/providencia culture

A

Swarming

30
Q

Proteus/providencia lactose

A

Negative

31
Q

Proteus/providencia pathogenicity

A

Saprophytic

Sometimes UTI

32
Q

Proteus/providencia subspecies

A

P. Vulgaris
P. Mirabilis
P. Morganii
Prov. Stuartii

33
Q

Yersinia species

A

Y. Pestis
Y. Pseudotuberculosis
Y. Entericolitica
Y. Ruckeri

34
Q

Y. Pseudotuberculosis habitat, morphology and flagella status

A

Hab: intestine, water
Morph: coccoid rods
Flagella in 22 degrees

35
Q

Y. Pseudotuberculosis culture

A

Not fastidious
Optimal temp 30
Small colonies
Lactose negative

36
Q

Y. Pseudotuberculosis

Antigens and pathogenicity

A 
O antigen (8)
H antigen (5)

Rodents
Turkey, pheasant, quail (septicemia, abscesses)
Cattle, swine, sheep abortion
Human

37
Q

Y. Enterocolitica
Habitat
Morphology
Culture

A

Intestine, water

Middle sized rods

Optimal temp: 22
Na-oxalate + bile salts

38
Q

Y. Enterocolitica antigens

Pathogenicity

A 
O antigens (34)
O9 =cross reaction with brucella

H = 20

Hu= GI infection, abscesses

Swine, rabbit = Int mesenterial ln, resembles tuberculosis

39
Q

Y. Ruckeri

Biochem and pathogenicity

A

Inactive

Trout septicemia,kidney (red mouth disease)

40
Q

Serratia habitat culture pathogenicity

A

Soil, water
Lower optimal temp, pigment
Saprophytes

41
Q

S. Marcescens

A

Food contamination

42
Q

Edwardsiella
Habitat
Culture
Pathogenicity

A

Intestine
Fastidious (vit and aa needed)
E. Tarda = eel
E. Ictaluri = septicemia, ascites (catfish)

43
Q

Gram negative anaerobic rods
Habitat
Pathogenicity

A

Mucous membranes
Anaerobic gut flora

Facultative pathogens
Mm, wounds, abscesses
Mixed infections

44
Q

Gram negative anaerobic rods genera

A

Fusobacterium
Bacteroides
Dichelobacter
Prevotella

45
Q

Fusobacterium
Morphology
Culture

A

Polymorph, no flagella
Fastidious (protein, yeast extract)
CO2

46
Q

Fusobacterium
Biochemistry
Resistance
Pathogenicity

A

Inactive, gelatins, casein
Weak
Facultative pathogenic
Anaerobic wounds, abscesses, gangrene

47
Q

Fusobacterium species

A

F. Necrophorum
Subsp necrophorum
Subsp funduliforme
F. Nucleatum

Bacteriology (13 decks)

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