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Bacteriology > Lab 6 Salmonella, Citrobacter, Shigella, Proteus, Providentia, Yersinia, Serratia, Edwardsiella, anaerobic Gram-negative rods > Flashcards

Lab 6 Salmonella, Citrobacter, Shigella, Proteus, Providentia, Yersinia, Serratia, Edwardsiella, anaerobic Gram-negative rods Flashcards

(47 cards)

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1
Q

Salmonella species

A

Only two:
S. Enterica
S. Bongori

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2
Q

Within s. Enterica

A

6 subspecies

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3
Q

Within subspecies

A

Serovars

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4
Q

Salmonella habitat

A

Gut
Sewage
Water
Feed

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5
Q

Salmonella morphology

A

Rod, flagella

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6
Q

What salmonella do not have flagella

A

S. Gallinarum

S. Pullorum

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7
Q

Salmonella culture

A

Not fastidious
Low selectivity: MacConkey, Drigalski
Medium selectivity: brill green phenol red, XLD, rambach agar

Selective enrichment
Na-selenite, cys
Na-tetrathionate, bile salts

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8
Q

Salmonella is lactose

A

Negative

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9
Q

Salmonella on rambach agar

A

Pink colonies

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10
Q

Salmonella H2S

A

Positive

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11
Q

Salmonella antigens

A

Kauffmann-white system
O = 35
Vi = K, S.typhi, s. Dublin
H = specific, non-specific, monophasic, diphasic

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12
Q

Salmonella only human

A

S. Typhi

S. Paratyphi A B C, S. Sendai

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13
Q

S. Typhi

A

Human typhoid fever

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14
Q

S. Paratyphi, S. Sendsai

A

Human paratyphoid

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15
Q

Salmonella only animals

A

S. Typhisuis

S. Gallinarum

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16
Q

S. Typhisuis

A

Swine typhoid

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17
Q

S. Gallinarum (pullorum)

A

Fowl paratyphoid

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18
Q

Salmonella human and animal

A

S. Enteritidis
S. Typhimurium
S. Thompson
S. Choleraesuis

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19
Q

Citrobacter habitat

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A

Mammals, birds, gut, water, environment

20
Q

Cireobacter lactose

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A

Negative

Late positive

21
Q

Citrobacter pathogenicity

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A

Saprophytic

Sometimes secondary pathogen

22
Q

Shigella habitat

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A

Intestine of primates, sewage

23
Q

Shigella flagella

24
Q

Shigella culture

25
Shigella lactose
Negative
26
``` Shigella S. Dysenteriae S.flexneri S. Sonnei S. Boydii ```
Human bacillary dysentery
27
Shigella mannitol and H2S
Negative both
28
Proteus/providencia habitat
Intestine Urine Water Rotting materials
29
Proteus/providencia culture
Swarming
30
Proteus/providencia lactose
Negative
31
Proteus/providencia pathogenicity
Saprophytic | Sometimes UTI
32
Proteus/providencia subspecies
P. Vulgaris P. Mirabilis P. Morganii Prov. Stuartii
33
Yersinia species
Y. Pestis Y. Pseudotuberculosis Y. Entericolitica Y. Ruckeri
34
Y. Pseudotuberculosis habitat, morphology and flagella status
Hab: intestine, water Morph: coccoid rods Flagella in 22 degrees
35
Y. Pseudotuberculosis culture
Not fastidious Optimal temp 30 Small colonies Lactose negative
36
Y. Pseudotuberculosis | Antigens and pathogenicity
``` O antigen (8) H antigen (5) ``` Rodents Turkey, pheasant, quail (septicemia, abscesses) Cattle, swine, sheep abortion Human
37
Y. Enterocolitica Habitat Morphology Culture
Intestine, water Middle sized rods Optimal temp: 22 Na-oxalate + bile salts
38
Y. Enterocolitica antigens | Pathogenicity
``` O antigens (34) O9 =cross reaction with brucella ``` H = 20 Hu= GI infection, abscesses Swine, rabbit = Int mesenterial ln, resembles tuberculosis
39
Y. Ruckeri | Biochem and pathogenicity
Inactive | Trout septicemia,kidney (red mouth disease)
40
Serratia habitat culture pathogenicity
Soil, water Lower optimal temp, pigment Saprophytes
41
S. Marcescens
Food contamination
42
Edwardsiella Habitat Culture Pathogenicity
Intestine Fastidious (vit and aa needed) E. Tarda = eel E. Ictaluri = septicemia, ascites (catfish)
43
Gram negative anaerobic rods Habitat Pathogenicity
Mucous membranes Anaerobic gut flora Facultative pathogens Mm, wounds, abscesses Mixed infections
44
Gram negative anaerobic rods genera
Fusobacterium Bacteroides Dichelobacter Prevotella
45
Fusobacterium Morphology Culture
Polymorph, no flagella Fastidious (protein, yeast extract) CO2
46
Fusobacterium Biochemistry Resistance Pathogenicity
Inactive, gelatins, casein Weak Facultative pathogenic Anaerobic wounds, abscesses, gangrene
47
Fusobacterium species
F. Necrophorum Subsp necrophorum Subsp funduliforme F. Nucleatum

Bacteriology (13 decks)

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