Lab 9 Brucella, Pseudomonas, Burkholderia, Aeromonas, Francisella, Bordetella, Moraxella

Question 1

Francisella habitat and morphology, resistance

Answer 1

Rodents, arthropods, environment

Coccoid rods, thin capsule

R: weak but good survival in cold

Round thin rats are weak but survive the cold in sanFRAN

Question 2

Francisella stain, culture

Answer 2

Gr-, bipolar
Aerobic, fastidious
Additives: protein, Cys, Yeast extract

Francis-Blood agar
Cys-Glu-Rabbit serum/egg

Question 3

Francisella biochemistry

Answer 3

Cat +, Oxi -
Glycerol
Pos: F. tularensis ssp tularensis and mediasiatica
Neg: F. tularensis ssp holarctica

Question 4

Francisella antigens, cross reactions with?

Answer 4

Uniform

Cross reactions with brucella and Y. pestis

Question 5

F. tularensis ssp. tularensis and ssp holarctica

Answer 5

Rodents, Ca, Fe, Bo, Ov, Su, Hu: tularaemia

Question 6

Bordetella habitat, morphology and stain, culture

Answer 6

Resp mm of mammals and birds (alle puster bordet)

Rods

Gr-

Obligate aerobic !!
Needs air, is found in resp mm!

Question 7

Bordetella biochemistry

Answer 7

Cat +
Oxi +!
Cannot utilize CH

Question 8

Bord. pertussis

Answer 8

Human whopping cough (kikhoste)
DIPERTE: vax
Diphteria (Coryne)
Pertussis (Bordetella)
Tetanus (Clostridium)

Question 9

Bord. parapertussis

Answer 9

Hu parapertussis

Question 10

Bord. bronchiseptica

Answer 10

Fimbria, growth on media containing only CITRATE
Selective isolation: CV, bile salts, penic, nitrofurantoin
Weak resistance
Su atrophic rhinitis (w/ P. multocida)
Ra pneumonia
Ca distemper complication
Fe bronchitis, bronchopneumonia

Question 11

Bord. avium

Answer 11

Poultry resp tract
Capsule, flagella, fimbria
Simple culture
Resp disease of turkey, a lot like Coryza

Question 12

Moraxella habitat, morphology, resistance

Answer 12

Mucous membrane of upper resp tract, conjunctiva and genitals

Coccoid rods, capsule, in pairs

R: weak

Question 13

Moraxella staining, biochemistry

Answer 13

Gr -

Cat +
Oxi +

Question 14

Mor. lacunata

Answer 14

Hu kerato-conjunctivitis

Question 15

Mor. bovis

Answer 15

Rods in pairs, fimbria
Fastidious culture, blood agar w/ beta hemolysis
Proteases, hyaluronidase, fibrinolysin
Infectious kerato-conjunctivitis of Bo: PINK EYE

Question 16

What causes pink eye of Bo:

Answer 16

Moraxella bovis

Question 17

Mor. ovis

Answer 17

Ov, calf kerato-conjunctivitis, nasal cavity

Cocci in pairs

Question 18

Brucella habitat, morphology, resistance

Answer 18

Host body, mucous membrane of genitals
Can’t survive in environment
Monospecies system/Multispecies system
B. melitensis abortus 1, 2, 3 / B. abortus 1, 2, 3

Coccoid rods, facultative IC
R: fair

Question 19

Brucella staining and culture

Answer 19

Gr-
Köster staining (safranin, H2SO4, methylene blue) A type of modified Ziehl-Neelsen

Obligate aerobic: NEEDS OXYGEN
Fastidious
Requires CO2 (10-15%) - brus har kullsyre
S-R colonies

Selective culture: polymyxin, bacitracin, cyclohexamide
Lab animals

Question 20

Brucella biochemistry, antigens

Answer 20

Cat +
Oxi +
UREASE POS

Ag:
S type: B. melitensis, B. abortus, B. suis cross reaction
R type: B. ovis, B. canis cross reaction
Cell wall A and M polysacch: mel, abo, suis

Differentiation of species:
Phage susceptibility, oxidative metabolism, natural host
Biotype differentiation:
CO2 demand, H2S, Fuchsin/thionin tolerance, agglutination

Question 21

Bruc. melitensis

Answer 21

Ov/Cap, testicle dilation (orchitis) dangerous!!

Notifiable

Question 22

Bruc. abortus

Answer 22

Bo

Question 23

Bruc. suis

Answer 23

Su, Hare, reindeer, rodents

Question 24

Bruc. pinnipediales
B. ceti
B. ovis
B. canis
B. neotomae
B. microti

Answer 24

Seal, walrus
Whale, dolphin
Ov
Ca
Rat
Vole

Question 25

Pseudomonas/Burkholderia | Habitat, morphology, resistance

Answer 25

Soil, water, plants, hospital environments (Except B.mallei) ``` Flagellated rods (except B. mallei) Many factors, enzymes and toxins that enhance virulence and make them resistant to commonly used Abs ```

Question 26

Pseudomonas/Burkholderia | Staining, culture

Answer 26

Not fastidious Obligate aerobic: NEEDS OXYGEN Pigment production Glycerol

Question 27

Burk. mallei

Answer 27

GLANDERS No flagella, irregular staining, no pigment production Weak resistance, Ew, Fe, Hu malleus

Question 28

Burk. pseudomallei

Answer 28

Soil, water, tropics Flagella! Glycerol agar, large colonies, forms layer in broth All mammals incl Hu excl Eq: melioidosis

Question 29

Pseudomonas aeruginosa | Characteristics

Answer 29

Higher resistance, can live everywhere (water, sewage, gut) 1 polar flagellum, polar fimbria Simple culture, 5-42C, beta hemolysis, odor and pigments --> GREEN COLOUR Susceptible: dehydration, heat or disinfection Resistant: quaternary NH4 salts, antibiotics

Question 30

Pseudomonas aeruginosa | Pathogenicity

Answer 30

``` Facultative pathogenic, local suppuration Bo, Eq: mastitis, metritis Ov: dermatitis Ca: otitis externa Fur animals: septicaemia Hu: nail and oral infection ```

Question 31

Pseudomonas anguilliseptica

Answer 31

Inactive biochemistry | Salmon, eel: septicaemia, keratitits, hemorrhages

Question 32

Aeromonas habitat, morphology, culture, groups

Answer 32

Water, sewage, gut Rods, flagellated Simple culture, aerobic/facultative anaerobic ``` Groups: Mesophilic: A. hydrophila = moderate temp Psychrophilic: A. salmonicida = cold temps ```

Question 33

Aero. hydrophila

Answer 33

Hemolysis, optimal temp 28 Resembles E.coli: sometimes gas is prod Colonise farm animals Snake, frog, fish: septicaemia

Question 34

Aero. salmonicida

Answer 34

Small coccobacillus, NO FLAGELLA 20C, dark brown pigment FURUNCULOSIS in Salmonidae