Lab Exam

Question 1

What is the goal of microscopy

Answer 1

To create a magnified image of objects too small to be seen with the eye alone.

Question 2

Uses a combination of glass lenses and light to view the specimen.

Answer 2

Brightfield microscope

Question 3

What are microscopes often used to study?

Answer 3

Bacteria
Cells
Tissues

Question 4

Fields of study where a microscope is a necessary tool include:

Answer 4

Cytology- study of cells
Histology- study of tissues
Pathology- study of disease

Question 5

What are the three factors that determine the quality of a microscopic image?

Answer 5

Magnification- increase of image
Resolution- see fine detail
Contrast- ability to distinguish objects (cells) from the background.

Question 6

The amount of slide you are able to see through the eyepiece:

Answer 6

Field of view

Question 7

The overall enlargement of the image of a specimen:

Answer 7

Total magnification

Question 8

To calculate total magnification, multiply the ______________ with the magnification of the __________.

Answer 8

Magnification of the ocular lens (10X); objective lens

Question 9

Is what you want to visualize in the middle of the field of view?

Answer 9

NO you must move the slide around to find what you want to study.

Question 10

This is a:

Answer 10

Stage clip- used to hold the microscope slide in place

Question 11

These are:

Answer 11

Ocular lenses- can be adjusted to fit the space between your eyes.

Question 12

What does this knob do?

Answer 12

Adjusts the light level

Question 13

What factor affecting image quality is altered by adjusting the light source?

Answer 13

Contrast

Question 14

Focus is achieved by adjusting the:

Answer 14

Distance between the specimen and the objective lens

Question 15

This is a:

Answer 15

Focus knob

Question 16

This is the:

Answer 16

Coarse focus ring- used to locate the focus plane

Question 17

This is the:

Answer 17

Fine adjustment knob- used to fine tune the focus.

Question 18

This is the:

Answer 18

Upper stage adjustment knob- used to navigate in the sample. Upper moves the stage back and forth.

Question 19

This is the:

Answer 19

Lower stage adjustment knob- used to navigate the sample. Lower moves the stage left to right.

Question 20

The 100X lens is an _____ objective; a drop of __________ should be applied between the lens and the microscope slide

Answer 20

Oil; Immersion oil.
Both should be cleaned when changing back to the other objectives

Question 21

This is the:

Answer 21

Nose piece- rotates 4X, 10X, 50X, 100X objective.