Lab Exam 2

Question 1

E. coli gram + or -

Answer 1

neg

Question 2

what shape is e coli

Answer 2

rods

Question 3

can E. coli ferment glucose

Answer 3

yes

Question 4

catalase results of e. coli

Answer 4

pos

Question 5

oxidase results of e. coli

Answer 5

neg

Question 6

Can E. coli ferment lactose

Answer 6

yes

Question 7

can S. typhimurium ferment lactose

Answer 7

no

Question 8

what does S. typhimurium produce on selective media

Answer 8

H2S

Question 9

what does SS agar differentiates bacteria based on

Answer 9

ability to ferment lactose and reduce

sulfur (produce H2S)

Question 10

sugar is SS agar

Answer 10

lactose

Question 11

what indicator dye is in SS agar

Answer 11

neutral red

Question 12

if an organism can ferment lactose on SS agar what color does it turn

Answer 12

pink

Question 13

what is the pink precipitate in SS agar

Answer 13

bile salts

Question 14

what does black precipitate on SS agar mean

Answer 14

hydrogen sulfide

(H2S) production

Question 15

KIA slant; Yellow color = ___ production = ______

Answer 15

acid

fermentation

Question 16

KIA slants: Red color = _____ products = _____

Answer 16

alkaline

protein catabolism

Question 17

kia slant: butt is yellow

Answer 17

organism can ferment glucose.

Question 18

if kia slant is red can the organism ferment lactose

Answer 18

no

Question 19

Blue Simmons citrate agar, organism can or can’t use citrate?

Answer 19

can

Question 20

Green Simmons citrate agar, organism can or can’t use citrate?

Answer 20

can’t

Question 21

indole broth: If indole is present (+), the reagent will turn ____

Answer 21

red

Question 22

indole broth: If indole is not present (-), the reagent will turn ____

Answer 22

clear

Question 23

is Streptococcus agalactiae catalase positive or neg

Answer 23

neg

Question 24

is Streptococcus agalactiae oxidase pos or neg

Answer 24

neg

Question 25

is Enterococcus faecalis catalase pos or neg

Answer 25

neg

Question 26

is Enterococcus faecalis oxidase pos or neg

Answer 26

neg

Question 27

what are streptococci and enterococci shape and arrangement

Answer 27

cocci

chains or pairs

Question 28

what kind of hemolysis does S. agalactiae have

Answer 28

beta

Question 29

what kind of hemolysis does enterococcus have

Answer 29

non-hemolytic

Question 30

true or false: enterococcus are highly antibiotic resistant

Answer 30

true

Question 31

streptococci are generally _____ to salt while enterococci are generally ___ grow in high salt environments

Answer 31

sensitive

able to

Question 32

what does a yellow color indicate on a bile esculin slant

Answer 32

negative for esculin hydrolysis

Question 33

what does a black color indicate on a bile esculin slant

Answer 33

positive for esculin hydrolysis

Question 34

what does a bacticard strep test for

Answer 34

distinguishes between streptococcus and enterococcus

Question 35

how fast does bacteria divide

Answer 35

exponentially

Question 36

how many colonies should be on a plate for standard acceptable range for an accurate

Answer 36

30-300