Lab Final Flashcards
(37 cards)
In Citrate medium, what is the:
Nitrogen Source
Carbon
N=ammonium phosphate
C=sodium citrate
Bacteria must possess ________ to convert molecules into pyruvate.
citrate-permease
color and texture of citrate slant inoculated
Green
solid agar
Citrate test result is blue means?
Citrate is utilized
PH increases
test is +
Citrate color is Green and shows growth means?
Citrate is utilized
Inoculation not complete
test is +
Citrate color is Green and shows NO growth means?
Citrate is NOT utilized
Test is -
Malonate and citrate tests are associated with what chain of events
Kreb Cycle
Important enzyme in malonate test
Succinate dehydrogenaze
Important chemical reduction in malonate test
FADH—>FADH2
Regular color and texture of molonate broth
green liquid broth
If malonate broth is dark blue it means?
Malonate Utilized
Test is +
If Malonate broth has no color change or slightly yellow
No Malonate Utilized
Test is -
Control color and texture of Phenylalanine Deaminase test
Yellowish Goo
If Phenylalanine Deaminase test is green it means?
Phenylalanine Deaminase is present
Test is +
If Phenylalanine Deaminase test is Yellow it means?
Phenylalanine Deaminase is absent
Test is -
Organisms that produce Phenylalanine Deaminase remove ________ from Phenylalanine?
amino group
Reagent used in Phenylalanine Deaminase Test
FeCl3
Ferric Chloride
Product of Phenylalanine Deaminase reaction with FeCl3
Phenylpyruvic Acid
Reactions that use water to split comlex molecules
hydrolysis
What reagent is used in Starch Hydrolysis to help detect the presence or absence of starch
Iodine
Type and names of enzymes associated with starch hydrolysis
Extracellular
amylase & oligo-1,6 glucosidase
how do enzymes hydrolyze starch
breaking the glycosidic linkages between sugar subunits
End result of starch hydrolysis
Breaking down polysacharides into smaller units
In the starch hydrolysis test, what does a clearing around the growth indicate?
Amylase is present
Test is +
