Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

From Flashcardlet > Lab Midterm #1 > Flashcards

Lab Midterm #1 Flashcards

(56 cards)

Start Studying
1
Q

What are the basic categories of colony morphology

A 
shape
elevation
margin
texture 
color
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

How do you handle a spill of viable organisms in the lab?

A

use dry paper towel so it can then be sterilized.

Wipe down area with disinfectant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Can you drink the lab ethenol?

A

Shit no!!

It’s Denatured for heaven sake!!!

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is the most common growth type seen in slants

A

Filiform

dense and opaque with a smooth edge.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Name the main steps of the gram stain knowing that wait 30 seconds and rinse comes between each step

A
  1. Heat fixed smear
  2. Crystal Violet
  3. Iodine
  4. Alchohol (acetone)Rinse
  5. Safrinin
  6. Blot Dry
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Iodine is added as a mordant to enhance crystal violet staining by forming a __________________

A

Crystal Violet Complex

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What step in the gram stain makes it a differential?

A

Decolorization with acetone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is safrinin in the gram stain
What color does it make the cells
What cells are affected

A

Counterstain
Reddish-pink
Gram Negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

The peptidoglycan wall of the gram positive cells can withstand the acetone decolorizing because of

A

Teichoic acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Which gram stain wall has more lipid

A

Gram negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What kind of stain is a the gram stain

A

differential

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

name the steps of the acid fast stain leaving out the rinsing with water

A
  1. heat fixed smear
  2. Carbolfuchsin for (7min)
  3. Rinse with acetone
  4. Methylene blue dye
  5. blot Dry
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Primary stain of acid fast stain

A

Carbolfuchsin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the waxy substance located in the walls of acid fast organisms that gives them a higher affinity for the primary stain and resistance to decolorization by acid alchohol?

A

Mycolic Acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Used to decolorize nonacid-fast cells

A

acid alchohol

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Acid fast cells resist the decolorization of acid alchohol and thus stain what color from the carbolfuchsin?

A

Reddish-Purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Non-Acid fast cells are stained what color after being decolorized and then exposed to a counter stain? What is the name of the counter-stain?

A 
Blue
Methylene Blue (brilliant green may also be applied)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

the acid fast stain is a differential stain used to identify what genus of bacteria? What diseases can certain species of this genus cause?

A

Mycobacterium

Tuberculosis &Leprosy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What does CFU stand for?

A

Colony forming unit

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What streak method is used for suspected high density samples
Low density samples?

A

Quadrant

Zig Zag

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

A countable plate has about how many colonies?

A

30-300

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Procedure that allows microbiologists to estimate the population density in a liquid sample by plating a very dilute portion of that sample and counting the number of colonies is produces.

A

Standard Plate Count

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Series of controlled transfers down a line of tubes containing a known diluent.

Study These Flashcards
A

Serial Diution

24
Q

Type of media designed to inhibit the growth of some organisms while encouraging the growth of others

Study These Flashcards
A

Selective media

25
Type of selective media containing indicators to expose differences between
Differential media
26
Mannitol Salt agar is used to find what type of microorganism? What type of media is it? Explain what makes it this type of media?
Staphylococcus Aureus Selective and Differential High salinity of MSA allows most Streps to grow Aureus strand ferments the mannitol and turns yellow
27
What type of media is MacConkey Agar? | What type of microorganism does it isolate?
Selective and DIfferential | Isolates the Enterobacteriaceae based on ability to ferment lactose
28
In MacConkey Agar, what type of bacteria do Bile salts and crystal violet inhibit?
Gram-Positive
29
What type of bacteria will turn pink with McConkey agar?
Lactose fermenters
30
How does phenol red indicate whether or not fermentation has taken place due to the change in PH
Change in color
31
What type of medium is Eosin Methylene Blue Agar
Differential and Selective
32
What possible bacteria is EMB looking for?
Gram +/- | Coliform/non-coliform
33
What 2 components usually make up a stain?
solent (water/ethenol) | chromogen (benzene based)
34
The charged portion of a chromogen which alows it to act as a dye through ionic or covalent bonds between the chromogen and the cell.
Auxochrome
35
the auxochrome becomes positively charged as a result of picking up a hydrogen ion or losing a hydroxide ion
Basic Stain
36
Pond bacteria that are easy to identify because of their bluish green color and photosynthetic pigments
Cyanobacteria | blue-green algae
37
Form of locomotion in Sarcodines (ameoba)
Pseudopods
38
Typical life cycle of Protozoans
Trophozoite (vegetive) | cyst
39
Type of reproduction in Protists
transverse fission
40
Type of staining technique in the Capsule stain.
negative stain | Differential
41
Endospore outer covering is made of what
Keratin
42
Steps of Capsule stain
1. Drop of broth on slide 2. Drop of Congo Red 3. acetone 4. Safrinin
43
Endospore Stain Steps
1. Heat fix smear 2. Paper towel with malachite green over boiling water 3. continue step 2 for 10 minutes 4. Safrinin
44
Standard test for measuring the effectiveness of antimicrobics against pathogenic microorganisms
Kirby- Bauer test
44
Standard test for measuring the effectiveness of antimicrobics against pathogenic microorganisms
Kirby- Bauer test
45
Which medium is used in the Kirby Bauer test
Mueller-Hinton agar
46
What are the 3 resistance mechanisms used by microorganisms against antibiotics
Enzymatic destruction of the drug Altered target Poor uptake into the cell
47
Name the full classification of protozoans
Unicellular eukaryotic heterotrophic
48
If a sample of nucleic acid is pure, what will it read on the UV spectrophotometer Diluted? Protein Contamination?
Between 1.65-1.85 More than 2 Less than 1.6
49
Formula for DNA concentration
260nm/280nm
50
What operon is used in the bacterial transformation experiment
Arabinose
51
Used as an indicator of Gene transcription in experiment
Green fluorescent protein
53
Used as the vector to introduce the gfp genes into E. coli cells
Plasmid
54
Attachment site for RNA polymerase during transcription of the GFP gene on the pGLO plasmid
Arabinose promoter
55
Regulatory molecule for the arabinose molecule
DNA binding protein
56
Necessary for the pGLO plasmid to replicate inside the cell
Replication origin

From Flashcardlet (67 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions