Lab: Koch’s Postulates Flashcards
(24 cards)
What is the first step in analyzing new patient samples?
Select a new patient sample from the ‘Patient Sample’ rack.
Why is it important to streak the patient sample for isolation?
To isolate individual colonies for further analysis.
What should you predict seeing on the plate after incubation?
Various colony morphologies.
What are colony morphologies?
Distinct growth patterns found on the plate.
How many milk agar plates do you need for each colony morphology type?
One milk agar plate for each colony morphology type.
What should you do after labeling the milk plates?
Streak the plate for isolation with the appropriate bacterial colony from the TSA plate.
What is the purpose of comparing your milk plate results with the ‘Clinical Laboratory’ results?
To identify potential etiological agents.
What is the next step after selecting the milk plate that may contain the etiological agent?
Perform a gram stain.
What should you include in your laboratory notebook regarding results?
Draw and describe all the colony morphologies and gram stain slides from each day.
What should be included in the conclusion of your laboratory activity?
Identify the part of the laboratory activity that correlated to each step of Koch’s Postulates.
What should you explain in your conclusion about Koch’s Postulates?
Whether you were able to use Koch’s Postulates to isolate the etiological agent causing the outbreak.
Who was Robert Koch?
German physician and bacteriologist known for research on bacterial infections
He worked on anthrax, tuberculosis, cholera, typhus, malaria, and trypanosomiasis.
What prestigious award did Robert Koch receive?
Nobel Prize in Physiology or Medicine in 1905
Awarded for his contributions to the understanding of bacterial infections.
What bacterium is associated with anthrax?
Bacillus anthracis
Identified by Koch as the etiological agent of anthrax.
What are Koch’s Postulates?
Steps to identify the causative agent of a disease
Developed by Robert Koch through his work on anthrax.
List the four steps of Koch’s Postulates.
- The same microorganism is found in all infected hosts.
- The microorganism found in infected hosts is grown in pure culture.
- The pure culture microorganism is injected into healthy hosts.
- The same microorganism is isolated from the ‘healthy’ hosts which have shown signs and symptoms of the same disease.
What was the main focus of Koch’s work published in 1876?
Proving that Bacillus anthracis causes anthrax in livestock
This work laid the foundation for Koch’s Postulates.
True or False: Koch’s Postulates can be used to identify the etiology of any disease.
False
Koch’s Postulates are primarily applicable to infectious diseases caused by microorganisms.
Fill in the blank: The microorganism found in infected hosts is grown in _______.
pure culture
What is the significance of Koch’s Postulates in microbiology?
They provide a framework for identifying the causative agents of infectious diseases.
What type of agar is mentioned in the laboratory activity?
Milk agar
Used for understanding the selective and/or differential nature in the context of the experiment.
Why is Koch’s work on anthrax particularly remembered?
It led to the formulation of Koch’s Postulates.
What disease did Koch study that was causing epidemic proportions in livestock?
Anthrax
What is the purpose of simulating Koch’s Postulates in a laboratory activity?
To identify the etiology of a simulated disease.
