Lab: Sensitivity to Disinfectants: Disk Diffusion Flashcards
(13 cards)
What is the main purpose of chemical disinfectants?
To disinfect surfaces and materials in settings such as laboratories, hospitals, and homes.
What is the goal of the activity described in the text?
To determine which disinfectants control or kill certain bacteria.
What is the first step in the procedure for Day One?
Using a sterile swab, inoculate a petri plate with one of the available bacteria.
What should you do with the swab after inoculating the petri plate?
Return the swab to the paper wrapper and dispose of it in the red biohazard bag on the kill cart.
What is the purpose of dipping forceps into alcohol and passing them through a Bunsen burner flame?
To sterilize the forceps before picking up a sterile paper disk.
What should be done after placing a drop of disinfectant into a well of the spot plate?
Dip the disk into the liquid and tap off any excess disinfectant on the side of the well.
How many disinfectants should be tested according to the procedure?
Five disinfectants.
What should be recorded on Day Two after examining the petri plates?
Observations of the clearing around the disks.
What should be done with the petri plates after the experiment?
Dispose of them in the biohazard bins near the kill cart.
What two sections should be included in the laboratory notebook after the experiment?
RESULTS and CONCLUSION.
Fill in the blank: Chemical disinfectants are not effective against _______.
[certain bacteria].
True or False: The activity aims to identify the most effective disinfectants for the majority of bacteria.
True.
What should be discussed in the CONCLUSION section of the laboratory notebook?
Which disinfectants were the most and least effective for the majority of bacteria.
