Lab Practical Exam 2 Flashcards
3 categories of tests that can be performed on a urine sample
- Chemical analysis
- Direct observation (gross inspection)
- Microscopic examination of urine sediments
4 categories of urine sediments
- Casts
- Cells
- Crystals
- Mucus threads
Abnormal urinalysis results & possible causes: glucose present
- Possible dietary cause
- Possible disease cause
- Large meal consumed
- Uncontrolled diabetes mellitus
Abnormal urinalysis results & possible causes: high pH (> 8.0)
- Possible dietary cause
- Possible disease cause
- Diet rich in vegetables, dairy products
- Severe anemia
Abnormal urinalysis results & possible causes: high specific gravity (> 1.025)
- Possible dietary cause
- Possible disease cause
- Decreased fluid intake, loss of fluids
- Uncontrolled diabetes mellitus, severe anemia
Abnormal urinalysis results & possible causes: low pH (< 4.5)
- Possible dietary cause
- Possible disease cause
- High protein diet, cranberry juice
- Uncontrolled diabetes mellitus
Abnormal urinalysis results & possible causes: low specific gravity (< 1.010)
- Possible dietary cause
- Possible disease cause
- Increased fluid intake
- Severe renal damage
Abnormal urinalysis results & possible causes: protein present
- Possible dietary cause
- Possible disease cause
- High protein diet
- Severe anemia
Abnormal urine color & possible causes: clear to light yellow color
- Diet
- Drugs
- Disease
- Alcohol
- Phosphate, carbonate
- Uncontrolled diabetes mellitus
Abnormal urine color & possible causes: yellow orange to dark green color
- Diet
- Drugs
- Disease
- Carrots
- Antibiotics
- Bilirubin from obstructive jaundice
Abnormal urine color & possible causes: brown-black
- Diet
- Drugs
- Disease
- Rhubarb
- Antidepressants
- Melanin pigment from melanoma
Abnormal urine color & possible causes: brown
- Diet
- Drugs
- Disease
- Rhubarb
- Barbiturates
- Anemia or liver infections
Abnormal urine color & possible causes: dark wine
- Diet
- Drugs
- Disease
- Beets
- Anti-inflammatory drugs
- Hemolytic jaundice
Abnormal urine color & possible causes: green
- Diet
- Drugs
- Disease
- Green food dye
- Diuretics
- Bacterial infection
Abnormal urine color & possible causes: red to red brown
- Diet
- Drugs
- Disease
- Beets
- Laxatives
- Hemoglobine & urine
Abnormal urine color & possible causes: smoky red
- Diet
- Drugs
- Disease
- Beets
- Anticonvulsants
- Unhemolyzed RBCs from urinary tract
Amylase
- What is it
- What produces it
- What does it do
- Digestive enzyme
- Produced by salivary glands and the pancreas
- Breaks dietary starches down into more simple sugars
Amylase is secreted by the salivary glands and the pancreas.
It breaks down ____
Starch
Bile
- Where is it produced
- Where is it stored
- What is it composed of
- Produced by the liver
- Stored and concentrated in the gallbladder
- Composed of bile pigments, bile salts, and cholesterol
Bile salts
- Function
- Emulsifier
Biuret reagent
- What is it
- How are results exhibited
- Protein indicator
- Detects the presence of peptide bonds
- pale blue = negative for protein
- pale purple = positive for protein
- pink = shorter peptides present
Bromothymol blue
- What does it indicate
- How are results exhibited
- Chemical pH indicator
- Results
- blue = pH > 7
- yellow = pH < 7
Cholesterol
- What is it
- Molecular structure
- Lipid
- Over 20 carbon atoms configured into 4 rings
Fatty acids
- Molecular structure
- Polar or nonpolar?
- Long chains of carbon with hydrogen attached
- Nonpolar