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Tissue Engineering and Regenerative Medicine > Lecture 10 > Flashcards

Lecture 10 Flashcards

1
Q

What do mesenchymal cells do normally in normal development in the body?

A

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2
Q

What are embryonic stem cells? Where are they derived from?

A

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3
Q

Can the environment alter the cells? What does this mean for embryonic stem cells?

A

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4
Q

Define Somatic cell?

A

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5
Q

Define Nuclear?

A

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6
Q

Define Transfer?

A

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7
Q

What is the somatic cell nuclear transfer (SCNT) experiment? What did it show? What were the steps?

A

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8
Q

Why was Dolly the sheep a break through? What did this show?

A

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9
Q

How did they make Dolly?

A

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10
Q

What is the problem with SCNT?

A

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11
Q

What is the future potential of SCNT in humans?

A

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12
Q

What about SCNT in humans? Concerns?

A

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13
Q

What is a problem with SCNT in humans? What solved this?

A

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14
Q

What is the timeline so far with SCNT?

A

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15
Q

What are induced pluripotent stem cells (iPSCs)? When were they discovered? What was used?

A

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16
Q 
What are the comparisons between embryonic stem cells, Somatic Cell Nuclear Transfer and Induced Pluripotent Stem cells? 
		a. Level of potency 
		b. Resources needed 
		c. Cell Replacement
Healthy/ Disease
A

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17
Q

What was the rationale behind iPSCs?

A

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18
Q

How to test whether something is contributing to the maintenance of the embryonic stem cell phenotype?

A

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19
Q

How was this test used to work out which transcription factors lead to ESCs?

A

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20
Q

How did they narrow down the number of factors? What experiment was done?

A

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21
Q

What were the final number of factors? What were they?

A

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22
Q

What other potential ways to make iPSCs?

A

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23
Q

What do most people use though?

A

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24
Q

What is the method to make iPSCs?

A

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25
Q

How long does the process take? How to identify iPSCs?

A

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26
Q

How to check whether the cells are good? What can happen?

A

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27
Q

If healthy with no mutations then what need to check to validate the authenticity of iPSCs?

A

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28
Q

How to check whether a cell is pluripotent?

A

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29
Q

What are the different pluripotent markers to check for?

A

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30
Q

What is the methylation to check for?

A

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31
Q

How to check for differentiation? What are the two methods?

A

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32
Q

How to check whether the iPSCs can contribute to the germ line? What experiment was done?

A

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33
Q

Why are iPSCs useful?

A

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Tissue Engineering and Regenerative Medicine (17 decks)

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