Flashcards in Lecture 10 - Enzyme Mechanisms Deck (24):
Why are enzymes central to all biochemical processes?
Enzymes show greater substrate specificity, and have an optimal arrangement of catalytic groups for chemical reaction
Enzymes increased reaction rates but not reaction equilibria
Which enzyme catalyzes the polymerization of nucleotides to form DNA?
List the inorganic ions that serve as cofactors for enzymes
Fe2+ or Fe3+
Interaction between substrate and enzyme is mediated by what?
The same noncovalent forces that stabilize protein structure
Lowers energy of transition state making it easier to form
Transition state stabilization
Arranges atoms for optimal activity
Binding removes interactions with solvent (water)
Substrate binding changes conformation of enzyme
What kind of catalysis pushes or pulls a proton?
Acid – base catalysis
What kind of catalysis adducts or intermediates?
What kind of catalysis involves Lewis acid and redox agents?
Metal ion catalysis
Energy derived from ES interaction is called what?
Binding energy (ΔGB)
True or false? Enzymes use binding energy to achieve substrate specificity and to stabilize the transition states through noncovalent interactions.
What kind of bonds or interactions are considered non-covalent interactions?
True or false? The mechanism of enzyme binding is a "lock and key" mechanism.
False, it is NOT
True or false? Enzymes bind the transition state with a greater affinity than either substrates or products.
Transition state analogues are good ________ of enzymes
Class of enzymes involved in transferring electrons
Class of enzymes involved in the group transfer reactions
Class of enzymes involved in hydrolysis reactions
Class of enzymes involved in the addition of groups to double bonds or double bond formation by removing groups
Class of enzymes involved in transfer of groups within a molecule to give an isomer
Class of enzymes involved in the formation of C – C, C – S, C – O, and C – N bonds by condensation reactions coupled with ATP cleavage or similar cofactor