Lecture 12 - Enzyme Kinetics & Inhibition Flashcards
(4 cards)
0
Q
Maximum number of substrate molecules an enzyme molecule can convert to product per unit time
A
k_cat (turnover number)
1
Q
What’s the study of the rate at which some processes progresses is called?
A
Kinetics
2
Q
True or false? A small value of k_cat is better
A
False, LARGER not smaller.
3
Q
Steps to experimentally determine K_m and V_max
A
1) Keep E_t constant
2) Use [S1] and measure the product formed (V0)
3) Draw the best straight-line rough the points to estimate V_0
4) Repeat for other concentrations
5) Graph V_0 versus [S]
