Lecture 2 - Staining Cont. and Cytology Flashcards Preview

First Semester: Microscopic Anatomy > Lecture 2 - Staining Cont. and Cytology > Flashcards

Flashcards in Lecture 2 - Staining Cont. and Cytology Deck (30):
1

How many colors are in Hematoxylin - eosin (HE) stains?

Two (red and blue)

2

What stains red/pink in HE?

acidophilic, like cytoplasm

3

What stains blue/purple in HE?

Basophilic, like plasma cells, pancreatic exocrine cells, chief cells in stomach (arrows), neurons, chondroblasts, osteoblasts.
Essentially, cells that produce protein

4

Nucleus (DNA) and basal cytoplasm containing RER (RNA) are...
baso or acidophilic?

Basophilic

5

Apical cytoplasm with
zymogen granules (enzyme precursors) – enzymes - proteins is...
baso or acidophilic?

Acidophilic

6

What's another word for acidophilic?

Eosinophilic

7

Silver stain is used for

reticular fibers, nerve fibers

8

Two types of elastic fiber stain?

Weigert's elastic stain
Orcein stain

9

In silver stain, nerve fibers are what color?

Black

10

Was is Periodic Acid Schiff used for?

-stain for basement membrane and to localize carbohydrates (stain magenta)

-polysaccharides (carbohydrate polymers ) such as glycogen,

-mucosubstances such as glycoproteins, glycolipids and mucins in tissues

11

In a Pas stain, what color are goblet cells?

pink/purple

12

Goblet cells

secrete mucus to protect airways

13

How is lipid stain preparation different?

Don't use alcohol-will wash out lipids
Instead, use frozen sections and can skip the washing and embedding steps

14

Enzyme histochemistry example:
In Gomori’s method for alkaline phosphatase the brush border of proximal convoluted tubules in the kidney stain what color?

black

15

Which colors bind to what in fluorescence microscopy?

Blue fluorescence binding to nuclear DNA.

Green fluorescent dye binds to actin filaments

16

In polarized light microscopy, collagen fibers are what color?
What about elastic fibers and nuclei?

Bright red or yellow

Not detected

17

Using an extra step to attach a secondary antibody (indirect), makes the compound

more specific

18

Immunocytochemistry vs immunohistochemistry

Cyto - cell
Histo - tissue

19

Three basic constituents of a cell

-cytosol (hyaloplasm)
-organelles (cell organelles)
-cell inclusions (paraplasm)

20

Hyaloplasm

basic structureless cytoplasm; the best electron microscopes reveal no structure in it.

21

Cell organelles

the nucleus, mitochondria, the Golgi complex, ribosomes, the endoplasmic reticulum, lysosomes, the cytoskeleton (centriole, microtubules, actin and
myosin filaments, intermediate filaments), proteasomes.

22

Cell inclusions (paraplasm)

secretory granules, glycogen, lipid droplets, crystaloids,
pigments: melanin, lipofuscin, lutein

23

Multiple nuclei are present in what cells? (4)

skeletal muscle cells, osteoclasts, megakaryoblasts / megakaryocytes and in giant cells (found in pathological processes)

24

In eukaryotic cells, the genome is separated from the cytoplasm by a

double nuclear envelope

25

Is chromatin baso or acidophilic?

basophilic

26

Two types of chromatin?

Heterochromatin (inactive) vs. Euchromatin (active)

27

The nuclear envelope connects to

the RER

28

What's the purpose of nuclear pores?

allow passage of RNA, proteins into the cytoplasm

29

What's the purpose of the nucleolus?

to produce rRNA

30

Most euchromatic cells fall into these four categories:

-Neurons
-Hepatocytes (liver cells)
-Pancreatic acinar cells - digestion
-Sertoli cells