Lecture 24 Flashcards
(25 cards)
What are the 3 examples of ATP production?
- 1, 3-bisphosphoglycerate plus ADP yields 3-phosphoglycerate (glyco)
- phosphenolpyruvate plus ADP yields pyruvate (glyco)
- succinyl coA plus inorganic phosphate plus GDP yields succinate and coA and GTP (CAC)
What converts GTP to ATP?
nucleoside diphosphokinase.
How much energy does the Electron transfer from NADH to O2 release?
-220 kJ
How much energy does each proton pumped across inner membrane store?
21 kJ
How many protons are pumped in NADH?
10 (4 in c 1 & 2 and 2 in IV)
How many protons are needed to make ATP?
4
How many protons are pumped in FADH2
6 protons
WHat are the shuttles that move the electrons?
Glycerol 3-phosphate shuttle and malate aspartate shuttle
What is true about the inner membrane?
is impermeable to NADH and NAD+
Where are the shuttles located?
cytoplasm
What determines the rate of ox phos?
The level of ADP
What is respiratory control?
The regulation of the rate of oxidative phosphorylation by the ADP
What is true about aerobic cells?
Aerobic cells have 4-10x more ATP than ADP. Thus, respiratory control is
dependent on ADP as a substrate for phosphorylation and ATP production.
What is true about ATP production?
electron flow through the respiratory chain is tightly coupled to phosphorylation of ADP
What is true about oxygen consumption?
Oxygen consumption increases when ADP
levels are high. When ADP levels are low,
ATP levels are high, O2 consumption goes
down
What happens during uncoupling?
It generates heat to maintain body temperatures
What is true about uncouplers?
Are lipid-soluble aromatic weak acid and deplete proton gradient by transporting protons across the membrane
What is an example of uncouplers?
2,4 dinitrophenol
How much ATP is produced from the mito matrix?
2.5
How much ATP does FADH yield?
1.5
How much ATP is made from NADH in the cytoplasm?
1.5 or 2.5
How many protons does NADH pump?
10 electrons
How many protons make up 1 ATP?
3.33
How many protons does FADH2 pump?
6 protons
