Lecture 9/02.03.25 Flashcards
(16 cards)
What is polyacrylamide gel electrophoresis?
It is a way into looking at proteins. This works by molecules migrating through an electrical field through a gel matrix according to size, shape, and charge.
What is SDS-PAGE?
A gel that denatures proteins causing multimeric proteins to dissociate into individual subunits.
How does SDS-PAGE work?
It coats them with a negative charge and separates them into mass which then affects their migration rate
What is isoelectric focusing?
It separates proteins by charge and leaves native form. They work by reaching a net zero charge
What is true about enzymes?
They are powerful catalysts with high substrate specificity that increase biochemical reactions. Also, SUPER specific.
What do enzymes not do?
alter the rate of biochemical reactions and affect the energetic of the reaction
What do enzymes require?
Co factors (coenzymes or metals) for catalytic activity
What are enzymes?
Generally proteins and sometimes RNA (ribozymes)
What bonding do enzymes have?
noncovalent interactions
How do enzymes increase rates?
They increase the rate by lowering the activation energy to from substrate to product
What is a rate?
The velocity of reaction
What is the unit for rate?
M s-1
What is the formula for rate?
V= -delta A/delta t= delta B/delta t
What is the formula for second order reactions?
V=k[A][B]
What happens to the k when there is lower activation energy?
The k is higher
What happens to the k when the activation energy is high?
The k is lower
