Lecture 32 Flashcards
(16 cards)
What is HPTLC?
High Performance Thin Layer Chromatography, more accurate and reproducible than TLC
HPTLC allows quantitative analysis of complex mixtures with low solvent use, making it more sustainable.
What is the purpose of DNA barcoding?
Identifies plant species using conserved DNA regions and detects adulterants in herbal products
DNA barcoding is useful when only plant parts are available and is now used in herbal monographs for more rigorous identification.
List some examples of uses for DNA barcoding.
- Authenticating Pterocarpus wood
- Authenticating teas
- Authenticating blueberries
- Authenticating kiwifruit
What are the types of drug release?
- Immediate release
- Delayed release
- Controlled/Extended release
What are nano-delivery systems?
Systems that improve solubility, stability, and uptake, typically less than 1000 nm
Formulations include liposomes, polymeric nanoparticles, cyclodextrins, and phytosomes.
What is an example of a phytosome?
Rutin phytosome for liver inflammation
Phytosomes are lipid-phytochemical complexes that enhance bioavailability.
What is transdermal delivery?
Delivery method using electrospun nanofibers for wound healing
These nanofibers have antioxidant, bactericidal properties and provide controlled release.
What are the advantages of using zebrafish as a model organism?
- Transparent embryos
- High-throughput & low-cost
- Genome sequenced, human disease models
- Small sample required
What was a use case for chlorogenic acid in zebrafish?
Reversed oxidative damage caused by auranofin
Effects included rescue of jaw, tail, and pericardial edema in embryos.
What are in silico approaches used for?
Molecular docking to screen phytochemicals for potential targets
This method is cost-effective for lead discovery.
What is driving the rapid growth of natural product use in the cosmetics industry?
Sustainability
This growth presents employment opportunities for BPharmSc graduates.
What are the key advances in identification methods?
- DNA barcoding
- HPTLC
What are the key advances in formulations?
- Nanoparticles
- Phytosomes
- Nanofibers
What model is used for bioactivity and toxicity evaluation?
Zebrafish embryos
What in silico method is used for discovering new targets?
Molecular docking
In which applications are recent advances in natural products being utilized?
- Medicine
- Cosmetics
- Supplements
