Limitations of microbial growth - Midterm 1 Flashcards Preview

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Flashcards in Limitations of microbial growth - Midterm 1 Deck (12):
1

Limits of Spectrophotometer

Does not provide direct info on the concentration and the cell numbers

2

True or false: its possible to determine the concentration of a culture from the optical density alone.

False

3

Why do we use spectrophotometer?

to measure the density of a culture . it provides a good indication of whether the density of a culture is higher or lower than a different or previously measured culture

4

Limitation of Hemocytometer

counts all of the cells including the dead ones. what if we want to only know how many are alive?

5

Purpose of hemocytometer?

allowing someone to obtain an exact count of the number of cells/ml for a given culture.

6

Advantage of hemocytometer

more rapid than plating method and
very precise for determining the total number of cells/ml

7

Purpose of viable cell count?

Allows you to determine a viable cell count

8

Limitations of viable cell count?

takes a long time to do, one has to wait 24 hours to let colonies grow.

9

how does spectrophotometer work?

light source shines a beam of light through a sample and a photometer on the opposite side measures the intensity of light that is able to pass through the solution

10

at what wavelength do we measure the yeast cells with the spectrophotometer?

600nm

11

What is transmitted light?

light that passes through a sample

12

What is a spectrophotometer?

a device that measures the fraction of light that is able to pass through a solution