MIDTERM 2 Catalase Test Flashcards Preview

BIMM 121 > MIDTERM 2 Catalase Test > Flashcards

Flashcards in MIDTERM 2 Catalase Test Deck (10):
1

What is Superoxide Dismutase and its significance and problems?

Its an enzyme that protonates superoxide to form hydrogen peroxide. Hydrogen peroxide is also toxic

2

How is the hydrogen Peroxide Dealt with?

The enzyme catalase breaks apart the hydrogen peroxide into water and O2

3

Where can catalase be found?

its produced by and found in essentially all actively growing microbes capable of using oxygen for respiration

4

Where does catalase exist?

It exists close to the cell surface and attacks external hydrogen peroxide.

5

What cultures are most suitable for a catalase test and why?

Cultures not older than 24 hours because the enzyme seems most active during exponential growth.

6

How do you test for catalase?

add a couple of drops of 3% hydrogen peroxide directly on the colony and observe if there are bubbles

7

what can the catalase test distinguish?

It can distinguish between morphologically similar but metabolically dissimilar microorganisms like costridium and bacillus

8

Is bacillus catalase positive?

Yes

9

Is clostridia catalase negative?

yes

10

Positive Test for catalase?

Oxygen gas visible with eye
Weak if visible within microscope at 10x