Flashcards in MIDTERM 2 Oxygen Requirements Deck (10):
Special quality of facultative bacteria?
they have enzyme systems enabling them to utilize free oxygen or some alternative oxygen source, such as nitrate. they may prefer either an aerobic or anaerobic lifestyle or have no preference at all.
What test is used for oxygen requirements?
What is the significance of the thioglycolate medium?
used to determine the oxygen requirements for an organism
What is contained in the Thioglycolate medium?
3. sodium thioglycolate
4. Resazurin (dye)
Why is Thioglycolate used?
its a reducing agent that lowers its oxidation-reduction potential.
What is Resazurin and why is it used?
its a dye and its used to indicate presence of oxygen
What color does the dye turn into under presence of oxygen?
When inoculating our medium, why is it important to do it gently?
because it can shake and this causes more oxygen to be taken into the medium
How long do we incubate the thioglycolate tubes for?