Liver Enzymes

Question 1

Other name for Aspartate Aminotransferase

Answer 1

Serum glutamic-oxaloacetic transaminase (SGOT) or serum glutamate-oxaloacetate transaminase

Question 2

Transfer of an amino group between aspartate and alpha- keto acids where oxaloacetate and glutamate are the products

Answer 2

AST

Question 3

Involved in the synthesis and degradation of amino acids

Answer 3

AST

Question 4

AST is not liver-specific because it is widely distributed, highest activities in

Answer 4

Cardiac, Liver and Skeletal Muscles

Question 5

Predominant isoenzyme in the serum

Answer 5

Cytoplasmic AST

Question 6

Elevated when there is severe injury or damage to the cell (mitochondrial membrane is damaged allowing mitochondrial AST to elevate)

Answer 6

Mitochondrial AST

Question 7

After MI, AST levels begin to rise in __ hours,
peak (highest serum level) at __ hours and return
to normal in __ days

Answer 7

6 to 8 hours
24 hours
5 days

Question 8

T/F; AST is released to a greater degree in chronic
disorder of the liver with progressive damage to
the hepatocytes.

Answer 8

TRUE

Question 9

Uses malate dehydrogenase and monitors decrease in absorbance at 340 nm

Answer 9

Karmen Method

Question 10

Reduce, high, increased absorbance

Answer 10

NADH

Question 11

Oxidized, decreased absorbance

Answer 11

NAD

Question 12

What is the color of the product in Karmen Method?

Answer 12

Colorless

Question 13

Secondary/coupling/indicator enzyme, found in reagent in Karmen Method

Answer 13

Malate Dehydrogenase

Question 14

The activity of AST is __ to the product of secondary enzyme (MD)

Answer 14

Directly proportional

Question 15

Alanine Aminotransferase (ALT) is also known as

Answer 15

Serum glutamic-pyruvic transaminase (SGPT)

Question 16

• Transfer of an amino group between alanine and alpha-ketoglutarate where the product is pyruvate and glutamate
• Increased in hepatocellular disorders

Answer 16

ALT

Question 17

More liver-specific than AST

Answer 17

ALT

Question 18

Is a significant marker in the evaluation of the hepatic disorders, specifically acute inflammatory conditions

Answer 18

ALT

Question 19

ALT is used to:

Answer 19

1. Monitor the course of hepatitis treatment
2. Monitor the effects of drug therapy especially among px with chronic disease
3. Also used to screen blood donors

Question 20

More sensitive and specific for post-transfusion hepatitis and occupational toxic exposure

Answer 20

ALT

Question 21

Secondary enzyme for ALT measurement

Answer 21

Lactate Dehydrogenase

Question 22

The AST/ALT Ratio
Differentiates the cause of hepatic disord

Answer 22

De Ritis Ratio

Question 23

Non-viral origin; AST is higher than ALT

Answer 23

Ratio >1

Question 24

Viral in origin; ALT is higher than AST

Answer 24

Ratio <1

Question 25

coenzyme for aminotransferases. This will serve as a prosthetic group

Answer 25

Pyridoxal Phosphate (Vitamin B6)

Question 26

Major organ affected SGOT/AST

Answer 26

Heart, muscle, liver

Question 27

Substrate of SGOT/AST

Answer 27

Aspartic Alpha Ketoglutaric acid

Question 28

End products of AST

Answer 28

Glutamic acid + Oxaloacetic acid

Question 29

Color developer used in AST

Answer 29

2,4 DNPH

Question 30

Color intensifier for AST

Answer 30

0.4 N NaOH

Question 31

Methods used for AST

Answer 31

Reitman and Frankel

Question 32

Major affected organ for SGPT/ALT

Answer 32

Liver

Question 33

Substrate for SGPT/ALT

Answer 33

Alanine alpha Ketoglutaric acid

Question 34

End product of SGPT/ALT

Answer 34

Glutamic acid + Pyruvic acid

Question 35

Color developer for ALT

Answer 35

2,4 DNPH

Question 36

Color intensifier methods

Answer 36

0.4 N NaOH

Question 37

Methods for ALT

Answer 37

Reitman and Frankel

Question 38

NDPH means

Answer 38

Dinitrophenylhyradizine

Question 39

is a colorimetric method for transaminase measurement

Answer 39

Reitman and Frankel

Question 39

Toxic Hepatitis

Answer 39

Both (AST and ALT)

Question 40

Acute Myocardial Infarction

Answer 40

AST

Question 41

Wolff-Parkinson White Syndrome (involuntary contraction of muscle)

Answer 41

AST

Question 42

Trichinosis (Parasitic infection of T. spiralis

Answer 42

AST

Question 43

Chronic Alcoholism

Answer 43

Both (AST and ALT)

Question 44

Dermatomyositis (muscle)

Answer 44

AST

Question 45

Hepatic Cancer

Answer 45

Both

Question 46

Reye's syndrome (Liver)

Answer 46

Both

Question 47

Viral Hepatitis

Answer 47

Both

Question 48

Muscular Dystrophy

Answer 48

AST

Question 49

Acute pancreatitis

Answer 49

AST

Question 50

Catalyze the transfer of the y-glutamyl residue to y-glutamyl peptides to amino acids, H2O, etc.

Answer 50

Gamma-Glutamyltransferase (GGT)

Question 51

Uses for diagnosis hepatobiliary disorders and chronic alcoholism

Answer 51

GGT

Question 52

Most common donor of gamma-glutamyl peptide is

Answer 52

Glutathione

Question 53

A sensitive marker for the determination of ethanol intoxication

Answer 53

GGT

Question 54

Used as marker for occult alcoholism (hidden consumption)

Answer 54

GGT

Question 55

Assay for GGT

Answer 55

Szaz Assay

Question 56

The absorbance of __ in Szaz assay is measured at

Answer 56

p-nitroaniline 405-420