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MTAP 2 - CC2 (Trans-Enzymology) > M.I Profile (Cardiac Enzyme) > Flashcards

M.I Profile (Cardiac Enzyme) Flashcards

1
Q

Type of transferase involved in the storage of high-energy creatine phosphate in muscle cells

A

Creatinine Kinase (CK)

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2
Q

Not specific to the heart because it is widely distributed, with highest activities in skeletal muscle, heart, and brain

A

CK

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3
Q

Give the structure of CK

A

Dimeric enzyme composed of 2 monomer subunits (either M or B)

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4
Q

Also known as Forward Reaction method for CK

A

Tanzer-Gilvarg

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5
Q

Optimum pH of forward reaction

A

9.0

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6
Q

Also known as Reverse Reaction

A

Oliver Rosalki

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7
Q

Optimum pH of reverse reaction

A

6-8

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8
Q

Hemolysis can false __ CK activity

A

Increase

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9
Q

RBC has __ which is the same with CK activity

A

Adenylate Kinase (AK)

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10
Q

CK is inactivated by light and can cause false __

A

Decrease

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11
Q

Physical activity and IM injection can __ CK

A

Increase

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12
Q

Slowest mobility toward the anode

A

CK-3 / CK-MM / Muscle type

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13
Q

Major isoenzyme in striated muscle and normal serum of healthy individuals

A

CK-3 / CK-MM / Muscle type

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14
Q

2nd fastest to migrate toward the anode

A

CK-2 / CK-MB / Hybrid Type

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15
Q

Significant quantities are found in heart tissues.

A

CK-2 / CK-MB / Hybrid Type

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16
Q

Migrate fastest toward the anode

A

CK-1 / CK-BB / Brain Type

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17
Q

Highest concentration in CNS, GI tract and uterus (pregnancy)

A

CK-1 / CK-BB / Brain Type

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18
Q

How many percent of CK MB is found in the total CK?

A

> 6%

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19
Q

After MI, CK-MB __ levels to begin to rise within __ hours, peak at __ hours, and returns to normal levels within 48-72 hours

A

> 6%
4-8
24

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20
Q

The first enzyme to elevate in Myocardial Infarction

A

CK MB

21
Q

First protein/ marker or analyte to elevate during Myocardial infarction, faster than CK-MB

A

Myoglobin

22
Q

More helpful in diagnostic and improve the specificity of the test in terms of identifying the target organ or tisse

A

CK Isoenzyme

23
Q

CK BB low in blood but high in CSF indicates

A

CSF Leakage (BBB is damaged)

24
Q

CK Isoenzyme that is dominant in the blood

A

CK MM

25
Q

CK enzyme is combined/coupled with antibodies or lipoprotein

A

Macro-CK

26
Q

CK isoenzyme with antibodies (IgA or IgG)

A

CK BB

27
Q

CK isoenzyme with Lipoprotein

A

CK MM

28
Q

Seen or migrate between CKMM and CKBB

A

Macro-CK

29
Q
  • Bound to mitochondrial membrane *severe damage to the cell
  • Migrates cathodal and farthest to anode
A

Mitochondrial CK (CK-MI)

30
Q
  • Oxidoreductase that catalyzes the interconversion of lactic and pyruvic acids
  • Widely distributed, highest activities in heart, hepatic, skeletal muscle and RBC
A

Lactate Dehydrogenase (LD)

31
Q

Forward reaction in LD assay

A

Wacker Method

32
Q

Optimal pH for wacker method

A

8.3 to 8.9

33
Q

Reverse reaction of LD assay

A

Wrobleuski La Due

34
Q

Three times faster but more susceptible to substrate exhaustion (prolonged testing or substrate testing may have high probability of exhaustion

A

Wrobleuski La Due

35
Q

Optimal pH for Wrobleuski La Due

A

7.1 to 7.4

36
Q

Has greater affinity of H subunits (greater the subunit=greater affinity)

A

Alpha-hydroxybutyrate dehydrogenase (a-HBD)

37
Q

Represent LDH-1 (since it composed ONLY of numerous H subunit

A

Alpha-hydroxybutyrate dehydrogenase (a-HBD)

38
Q

After MI, LD begin to rise within __ hours, peak at 48-72 hours and remains elevated for __days

A

10-24
10

39
Q

Structure of LD

A

Tetramer and has 2 active subunits (M and H)

40
Q

Give the tissue and disorder: LDH 1 (HHHH)

A

Heart, RBC
MI, Hemolytic anemia

41
Q

Give the tissue and disorder: LDH 2 (HHHM)

A

Heart, RBC
RI (Renal infarction), Megaloblastic anemia

42
Q

Give the tissue and disorder: LDH 3 (HHMM)

A

Lung, Spleen, Pancreas
Pulmonary embolism

43
Q

Give the tissue and disorder: LDH 4 (HMMM)

A

Liver
Hepatic injury

44
Q

Give the tissue and disorder: LDH 5 (MMMM)

A

Skeletal Muscle
Skeletal Muscle Injury

45
Q

T/F: LDH can also be a liver enzyme

A

TRUE

46
Q

LDH isoenzyme that is fastest and closest to the anode

A

LDH 1 (HHHH)

47
Q

The most predominant LD Isoenyzme in healthy individuals and greater with LDH 1

A

LDH 2 (HHHM)

48
Q

Shows in AMI and intravascular hemolysis (LDH 1 > LDH2)

A

Flipped Pattern

MTAP 2 - CC2 (Trans-Enzymology) (9 decks)

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