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MTAP 2 - CC2 (Trans-Enzymology) > Other Liver enzymes > Flashcards

Other Liver enzymes Flashcards

(46 cards)

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1
Q

Other name for Alkaline Phosphate

A

Alkaline orthophosphoric monoester phosphohydrolase

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2
Q

Catalyze the hydrolysis of various phosphomonoesters at an alkaline pH (around 9-10)

A

Alkaline Phosphate

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3
Q

Liberate inorganic phosphate from an organic phosphate ester with production of alcohol

A

Alkaline Phosphate

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4
Q

ALP requires what activator?

A

Magnesium

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5
Q

In healthy human sera, most ALP is derived from the

A

Liver and bones specifically OSTEOBLAST

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6
Q

For evaluation of hepatobiliary and bone disorders

A

ALP

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7
Q

Major tissue sources of ALP

A

Liver, Bones, Placenta, Intestine and Kidneys

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8
Q
  • Based on molar absorptivity and p-nitrophenol
  • Absorbance is measured at 405 nm (visible light spectrum
A

Bowers and McComb

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9
Q

Color of product in Bowers and McComb

A

Yellow

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10
Q

Bodansky, Shinoowara, Jones, Reinhart substrate and end product

A

S: b-glycero-phosphate
E: Inorganic PO4 and Glycerol

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11
Q

Bessy, Lowry and Brock, Bowers and McComb substrate and end product

A

S: P-nitrophenyl phosphate
E: P-nitrophenol (yellow)

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12
Q

King and Armstrong substrate and end endproduct

A

S: Phenyl phosphate
E: Phenol

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13
Q

T/F: among kids (10-puberty) is because the bone cell is affected by growth hormone surge and during bone growth, osteoblasts are active → ALP is released

A

TRUE

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14
Q

Fastest ALP isoenzyme and elevates in liver diseases; most anodic

A

Liver ALP

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15
Q

Two fractions of Liver ALP

A

Major liver (abundance)
Fast liver (a1) band (fast migration)

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16
Q

Heat labile (when temperature is increased, the activity is significantly decreased [seen at 56 deg C])

A

Bone ALP

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17
Q

Increase in bone dse., healing of bone fractures and physiologic bone growth

A

Bone ALP

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18
Q

Can be pathologic (PAGET’S dse or osteitis deformans) and physiologic (bone fracture because of the activity of osteoblast [repair process])

A

Increase Bone ALP

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19
Q

Most heat stable fraction and increase in pregnancy (16th-20th week of gestation)

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A

Placental ALP

20
Q

Associated or linked in malignancy or cancer

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A

Carcinoplacental ALP

21
Q

ALP isoenzyme which is the slowest; least anodic
- Slowest moving fraction, in blood groups B or O (secretors)

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A

Intestinal ALP

22
Q

Placental and Intestinal ALP are inhibited by

Study These Flashcards
A

Phenylalanine

23
Q

Bone ALP is inhibited by what chemical inhibitor

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A

3M urea reagent

24
Q

Chemical inhibitor for Bone and Liver ALP

25
Total ALP elevations by the liver or bone ALP is differentiated by
Heating of serum at 56degC for 10 mins
26
Heat stability characteristics
Placental>Intestinal >Liver >Bone (placental - most heat stable ; bone - most heat labile)
27
ALP residual activity is decreased up to >20%
Liver ALP
28
ALP residual activity is decreased to <20% (heat labile)
Bone ALP
29
- Found on lung, breast, and gynecological cancers, bone ALP co-migrator - Most heat-stable ALP (remains stable even after heating the serum sample at 65degC for 30 mins)
Regan ALP
30
Seen on adenocarcinoma of the pancreas and bile duct, pleural cancer
Nagao ALP
31
Reagent that can inhibit Regan ALP
Phenylalanine
32
Reagents that can inhibit Nagao
Phenylalanine, L-leucine
33
Acid Phosphatase (ACP) is also known as
Acid orthophosporic monoester phosphohydrolase
34
Catalyze the hydrolysis of various phosphomonoesters at an acid pH
ACP
35
Sources of Acid phosphatase
Prostate gland, RBC, platelets, and bones (osteoclast)
36
● Liberate inorganic phosphate from an organic phosphate ester with production of alcohol ● For evaluation of metastatic carcinoma of prostate
ACP
37
Now used as a tumor marker for prostate cancer since it is more specific than ACP
PSA (Prostate-Specific Antigen)
38
Prostatic ACP activity in cases of rape can only persist for how many days?
4
39
Inhibit specific prostatic ACP
L-tartrate ions
40
Inhibits red cell ACP
Formaldehyde and cupric ions
41
Substrate for quantitative end point
Thymolpthalein monophosphate
42
Substrate for Continuous monitoring
A-napthyl phosphate
43
Give the substrate and end product for Gutman and Gutman
S: Phenyl PO4 E: Inorganic phosphate
44
Shinowara substrate and endproducts
S: PNPP E: P-nitrophenol
45
Babson, Read and Phillips substrate and end products
S: Alpha naphtyl PO4 E: Alpha naphtol
46
Substrate and end product for Roy and Hillman
S: Thymolphthalein MonoPO4 E: Free thymolphthalein

MTAP 2 - CC2 (Trans-Enzymology) (9 decks)

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