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MTAP 2 - CC2 (Trans-Enzymology) > Pancreatic Enzymes > Flashcards

Pancreatic Enzymes Flashcards

1
Q

Amylase is also known as

A

Alpha-1-4-glucan-4-glucohydrolase

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2
Q

Catalyzes the breakdown of polysaccharides like starch and glycogen via alpha-1-6 branching linkages

A

Amylase

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3
Q

Major tissue source of Amylase

A

Pancreas, Salivary gland

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4
Q

Minor tissue source of Amylase

A

Adipose tissue, small intestine, skeletal muscles and fallopian tube

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5
Q

Increase amylase is seen on

A

Acute pancreatitis, renal failure, and parotitis

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6
Q

The fastest to elevate (2-12 hours rise after the onset of attack acute pancreatitis)

A

Amylase

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7
Q

Level of amylase in the circulation normalizes after

A

3-5 days

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8
Q

T/F: Amylase is NOT specific to acute pancreatitis since there are other source in other organs or glands (parotid/salivary gland)

A

TRUE

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9
Q

T/F: Increase levels of Amylase in the plasma, increase in the urine

A

TRUE

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10
Q

Smallest enzyme in the plasma, therefore, normally filtered by the renal glomerulus (present in urine)

A

Amylase

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11
Q

Amylase in urine is decreased since it is reabsorbed so amylase in plasma is increased

A

Renal failure

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12
Q

Salivary amylase

A

Ptyalin (fast moving) *s-type

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13
Q

Pancreatic amylase

A

Amylopsin (slow moving) *p-type higher level than s-type

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14
Q

Measures the disappearance of starch substrate
Starch-iodine complex (Dark Blue) → Decrease in color intensity

A

Amyloclastic

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15
Q

Alternative substrate for starch

A

Glycogen

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16
Q

Iodine + Glycogen will result in what color?

A

Mahogany Brown

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17
Q

Measures the appearance of the product

A

Saccharogenic

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18
Q

Measures the increasing color from the production of a product -chromogenic dye fragment

A

Chromogenic

19
Q

Coupling or several enzyme systems to monitor amylase activity

A

Continuous Monitoring

20
Q

Amylase activity is directly proportional to absorbance in what methods?

A

Chromogenic and Saccharogenic

21
Q

Amylase activity is inversely proportional to absorbance in what method?

A

Amyloclastic

22
Q

Lipase is also known as

A

Triaceylglycerol acylhydrolase

23
Q

● Hydrolyzes the ester linkages of fats to produce alcohols and fatty acids
● Used as a digestive enzyme

A

Lipase

24
Q

Hydrolysis of dietary glycerides in the intestine to 2-monoglyceride and fatty acids

A

Lipase

25
Q

Early and the most specific marker for acute pancreatitis

A

Lipase

26
Q

Is secreted by the pancreas and not affected by renal disorder and salivary gland dse

A

Lipase

27
Q

Major tissue source of Lipase

A

Pancreas

28
Q

Lipase is a large molecule that remains in the circulation for how many days?

A

7

29
Q

Elevation of pancreatitis starts after

A

6 hours

30
Q

Substrate used fro Cherry Crandall and Tietz method?

A

50% olive oil (triolein)

31
Q

Titrating agent used for Cherry Crandall and Tietz

A

0.4 NaOH

32
Q

Indicator in Cherry Crandall

A

Phenolpthalein

33
Q

Indicator in Tietz

A

Thymolpthalein + veronal

34
Q

Endpoint for Cherry Crandall and Tietz

A

Fatty acid (Oleic acid)

35
Q

End color for Cherry Crandall

A

Pink

36
Q

End color for Tietz

A

Blue

37
Q

More pure form of triglyceride and is also used as a substrate for lipase test

A

Triolein

38
Q

It is the reference method for test of Lipase

A

Cherry Crandall

39
Q

Hemolyzed sample is not allowed because it will inhibit hemoglobin and will cause false _

A

Decrease

40
Q

T/F: Higher the lipase activity, higher level of liberated triglyceride

A

TRUE

41
Q

Estimation of liberated fatty acids

A

Turbidimetric methods

42
Q

Triglyceride is hydrophobic/insoluble, more turbid sample is equal to

A

More light blocking

43
Q

The higher the lipase activity, the __ the absorbance

A

Lower absorbance (Inversely proportional)

MTAP 2 - CC2 (Trans-Enzymology) (9 decks)

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