Pancreatic Enzymes

Question 1

Amylase is also known as

Answer 1

Alpha-1-4-glucan-4-glucohydrolase

Question 2

Catalyzes the breakdown of polysaccharides like starch and glycogen via alpha-1-6 branching linkages

Answer 2

Amylase

Question 3

Major tissue source of Amylase

Answer 3

Pancreas, Salivary gland

Question 4

Minor tissue source of Amylase

Answer 4

Adipose tissue, small intestine, skeletal muscles and fallopian tube

Question 5

Increase amylase is seen on

Answer 5

Acute pancreatitis, renal failure, and parotitis

Question 6

The fastest to elevate (2-12 hours rise after the onset of attack acute pancreatitis)

Answer 6

Amylase

Question 7

Level of amylase in the circulation normalizes after

Answer 7

3-5 days

Question 8

T/F: Amylase is NOT specific to acute pancreatitis since there are other source in other organs or glands (parotid/salivary gland)

Answer 8

TRUE

Question 9

T/F: Increase levels of Amylase in the plasma, increase in the urine

Answer 9

TRUE

Question 10

Smallest enzyme in the plasma, therefore, normally filtered by the renal glomerulus (present in urine)

Answer 10

Amylase

Question 11

Amylase in urine is decreased since it is reabsorbed so amylase in plasma is increased

Answer 11

Renal failure

Question 12

Salivary amylase

Answer 12

Ptyalin (fast moving) *s-type

Question 13

Pancreatic amylase

Answer 13

Amylopsin (slow moving) *p-type higher level than s-type

Question 14

Measures the disappearance of starch substrate
Starch-iodine complex (Dark Blue) → Decrease in color intensity

Answer 14

Amyloclastic

Question 15

Alternative substrate for starch

Answer 15

Glycogen

Question 16

Iodine + Glycogen will result in what color?

Answer 16

Mahogany Brown

Question 17

Measures the appearance of the product

Answer 17

Saccharogenic

Question 18

Measures the increasing color from the production of a product -chromogenic dye fragment

Answer 18

Chromogenic

Question 19

Coupling or several enzyme systems to monitor amylase activity

Answer 19

Continuous Monitoring

Question 20

Amylase activity is directly proportional to absorbance in what methods?

Answer 20

Chromogenic and Saccharogenic

Question 21

Amylase activity is inversely proportional to absorbance in what method?

Answer 21

Amyloclastic

Question 22

Lipase is also known as

Answer 22

Triaceylglycerol acylhydrolase

Question 23

● Hydrolyzes the ester linkages of fats to produce alcohols and fatty acids
● Used as a digestive enzyme

Answer 23

Lipase

Question 24

Hydrolysis of dietary glycerides in the intestine to 2-monoglyceride and fatty acids

Answer 24

Lipase

Question 25

Early and the most specific marker for acute pancreatitis

Answer 25

Lipase

Question 26

Is secreted by the pancreas and not affected by renal disorder and salivary gland dse

Answer 26

Lipase

Question 27

Major tissue source of Lipase

Answer 27

Pancreas

Question 28

Lipase is a large molecule that remains in the circulation for how many days?

Answer 28

7

Question 29

Elevation of pancreatitis starts after

Answer 29

6 hours

Question 30

Substrate used fro Cherry Crandall and Tietz method?

Answer 30

50% olive oil (triolein)

Question 31

Titrating agent used for Cherry Crandall and Tietz

Answer 31

0.4 NaOH

Question 32

Indicator in Cherry Crandall

Answer 32

Phenolpthalein

Question 33

Indicator in Tietz

Answer 33

Thymolpthalein + veronal

Question 34

Endpoint for Cherry Crandall and Tietz

Answer 34

Fatty acid (Oleic acid)

Question 35

End color for Cherry Crandall

Answer 35

Pink

Question 36

End color for Tietz

Answer 36

Blue

Question 37

More pure form of triglyceride and is also used as a substrate for lipase test

Answer 37

Triolein

Question 38

It is the reference method for test of Lipase

Answer 38

Cherry Crandall

Question 39

Hemolyzed sample is not allowed because it will inhibit hemoglobin and will cause false _

Answer 39

Decrease

Question 40

T/F: Higher the lipase activity, higher level of liberated triglyceride

Answer 40

TRUE

Question 41

Estimation of liberated fatty acids

Answer 41

Turbidimetric methods

Question 42

Triglyceride is hydrophobic/insoluble, more turbid sample is equal to

Answer 42

More light blocking

Question 43

The higher the lipase activity, the __ the absorbance

Answer 43

Lower absorbance (Inversely proportional)