M Flashcards
(10 cards)
1
Q
Restriction site
A
Sequence of DNA that is recognized and cut by a restriction enzyme
(Involved in process of gene being inserted into plasmid)
2
Q
Gel electrophoresis
A
Allows for separation of Nucleic acids in a DNA mixture by size
Fragments of - charged nucleotides are pulled towards anode
Smallest pieces get pulled farther
3
Q
PCR
3 steps and purpose
A
- Denaturation-Separation of Template strands
2.Annealing-Primers bind to the 3’ of each single strand
3.Extension- Taq polymerase extends the primers using the TS as a guide
Amplifies/makes copies of specific DNA region
4
Q
Primer
A
5
Q
Primer vs probe
A
6
Q
Taq polymerase
A
7
Q
RT-PCR
A
8
Q
RT-PCR
A
9
Q
In situ hybridization
A
10
Q
DNA microarray
A
