M3 L10

Question 1

ResD

Answer 1

F plasmid recombinase
- acts at fcr near oriV

Question 2

cer site

Answer 2

ColE1 site for site-specific multimer resolution recombination
-35bp

Question 3

XerCD

Answer 3

ColE1 host-encoded chromosomal recombinase
- acts at cer sites for multimer resolution

Question 4

ColE1 host control mechanism

Answer 4

addiction system
- colicinE1
- imm - immunity to Colicin
cells that do not produce immunity protein die

Question 5

F plasmid host control mechanism

Answer 5

• hok/sok
• ccdAB

Question 6

OriT

Answer 6

Origin of transfer - where DNA is nicked and transferred during conjugation

Question 7

tra

Answer 7

conjugal plasmids only
- encode mating pore and DNA mobilisation functions for conjugative transfer

Question 8

mob

Answer 8

mobilisable plasmids only
- relaxase required for mobilisation
- acts at oriT

Question 9

temperate phage

Answer 9

phage that can undergo lytic or lysogenic pathways

Question 10

pathogenicity

Answer 10

the ability of a microbe to cause disease

Question 11

virulence

Answer 11

the degree of pathogenicity of strains