M3 L9 Flashcards
(10 cards)
Mobile genetic elements
the agents of horizontal gene transfer
pangenome
core genome + accessory genome
core genome
-located on chromosome(s)
- essential genes conserved in all members of a species
accessory genome
-located on mges
- non-essential genes of variable occurrence through a bacterial species
- acquired through horizontal gene transfer
important plasmid-encoded phenotypes
-antibiotic and bacteriocin synthesis (and resistance)
- virulence
- symbiotic nitrogen fixation
things plasmids must do
- replicate
- keep host happy
- segregate
- control host
- spread
oriV
origin of vegetative replication
- where DNA rep begins
rom
inhibitor protein gene required for copy number control
- encodes rop protein
RepF1A
determines vegetative replication in F plasmids
- includes oriV
- allows 1-2 plasmids per cell
par
partitioning loci