Methods

Question 1

LISS method of enhancement

Answer 1

decreases ionic strength

Question 2

PEG method of enhancement

Answer 2

removing water and concentrating antibody

Question 3

Glycoprotein blood groups

Answer 3

HLA system

Question 4

Glycolipid blood groups

Answer 4

ABH, Lewis, Ii, and P systems

Question 5

Protein blood groups

Answer 5

Rh, M, N

Question 6

greatest to least amount of H in blood groups

Answer 6

O > A2 > B > A2B > A1 > A1B

Question 7

What does Dolichos biflorus aggluntinate?

Answer 7

A1 or A1B cells

Question 8

What does Bandeiraea simplicifolia agglutinate?

Answer 8

B cells

Question 9

What does Ulex eurpoaeus agglutinate?

Answer 9

O cells/H-antigen (anti-H)

Question 10

ABO antigens can be detected on red cells of embryos as early as:

Answer 10

5 to 6 weeks of gestation

Question 11

adult levels of ABO antigens are generally observed by age

Answer 11

2 - 4 y/o

Question 12

FUT1

Answer 12

H gene

Question 13

FUT# that specifically fucosylates type 2 chain oligosaccharides on red cell glycoproteins and glycolipids to form type 2 chain H

Answer 13

FUT1

Question 14

FUT2

Answer 14

Secretor gene

Question 15

FUT# that recognizes type 1 chain precursors to form type 1 chain H and Leb antigens

Answer 15

FUT2

Question 16

FUT3

Answer 16

Lewis gene

Question 17

R1

Answer 17

DCe

40% W, 17% B, 70% A

Question 18

R2

Answer 18

DcE

15%

Question 19

R0

Answer 19

Dce

5% W, 45%B

Question 20

RZ

Answer 20

DCE

<0.01%

Question 21

r

Answer 21

ce

35% W, 25% B

Question 22

r’

Answer 22

Ce

2%

Question 23

r’’

Answer 23

cE

1% W, <0.01%B

Question 24

ry

Answer 24

CE

<0.01%

Question 25

Rh1 ISBT

Answer 25

D | 85 W, 92B

Question 26

Rh2 ISBT

Answer 26

C | 70 W, 30 B

Question 27

Rh3 ISBT

Answer 27

E | 30%

Question 28

Rh4 ISBT

Answer 28

c (little) | 80 W, 95 B

Question 29

Rh5 ISBT

Answer 29

e | 98%

Question 30

Rh6 ISBT

Answer 30

ce or f | 65 W, 27B

Question 31

Rh7 ISBT

Answer 31

Ce or rh(i) | 70 W, 30B

Question 32

Rh8 ISBT

Answer 32

Cw | low, 2%W

Question 33

Rh9 ISBT

Answer 33

Cx | low

Question 34

Rh10 ISBT

Answer 34

V | 30% B

Question 35

Rh11 ISBT

Answer 35

Ew | low

Question 36

Rh12 ISBT

Answer 36

G | 85%

Question 37

Strength of D (haplotypes in order)

Answer 37

R2R2, R1R2, R2r, R1r

Question 38

A-subgroup that shows mixed field in forward type

Answer 38

A3

Question 39

A-subgroup that shows reactivity with Anti-A,B, typically no reactivity with Anti-A

Answer 39

Ax

Question 40

B subgroup that shows mixed field in forward type

Answer 40

B3

Question 41

B subgroup that shows 0/wk with Anti-B and 0/2+ with Anti-A,B

Answer 41

Bx

Question 42

K KEL1

Answer 42

9 W, 2 B

Question 43

k KEL2

Answer 43

99%

Question 44

Kpa KEL3

Answer 44

2 W, <0.01 B

Question 45

Kpb KEL4

Answer 45

100%

Question 46

Ku KEL5

Answer 46

100

Question 47

Jsa KEL6

Answer 47

0.01 W, 20 B

Question 48

Jsb KEL7

Answer 48

99%

Question 49

IgM % of total serum concentration

Answer 49

5-10%

Question 50

IgG % of total serum concentration

Answer 50

80%

Question 51

IgM serum half-life

Answer 51

5-6 days

Question 52

IgG serum half-life

Answer 52

23 days

Question 53

apheresis donor rxn: chills, parasthesias, nausea/vomiting, cramping, cardiac arrhthymia

Answer 53

citrate reaction

Question 54

apheresis donor rxn: respiratory distress, chest pain, collapse

Answer 54

air embolism

Question 55

apheresis donor rxn: flushing, hives, cough, respiratory distress

Answer 55

anaphylactic reaction

Question 56

apheresis donor rxn: back pain, shock, cardiac arrest

Answer 56

hemolysis

Question 57

RBC life span

Answer 57

120 days

Question 58

granulocytes life span

Answer 58

half life = 7.2 hours

Question 59

lymphocytes life span

Answer 59

4 - 10 years

Question 60

monocytes life span

Answer 60

half life = 71 hours

Question 61

platelets life span

Answer 61

8-10 days