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Flashcards in Micro: Things I don't know Deck (101)
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1

HPV
1. genome
2. capsid
3. envelope

1. small circular dsDNA
2. icosahedral capsid: self assemble
3. non-enveloped: contributes stability of virus on skin and fomites

2

L1

protein capsid of HPV used for vaccine

3

laryngeal papilloma

HPV 6 and 11
infants/young children
benign warts in respiratory tract
complication: respiratory distress leading to death
Tx: multiple surgeries
prevention: remove genital warts in pregnancy

4

epidermodysplasia verruciformis

HPV infection in someone with inherited defect in cellular immunity
warts on face, trunk, limbs throughout life and non-metastatic tumors

5

HPV genital warts

condyloma acuminata
condyloma plana

6

SPI

subclinical papilloma infection
HPV infection that can lead to CA

7

5% acetic acid

HPV
brush on infected area to detect SPI: turns warts white
reveals dysplasia: use to take colposcopy

8

pap smear

HPV
detects koilocytotic squamous epithelial cells

9

cofactors in the development of cervical cancer in women with HPV

smoking
co-infection with herpes simplex

10

Dx of HPV

CANNOT be grown in cell culture
1. clinical appearance
2. abnormal Pap, colposcopy to look for dysplasia
3. HPV DNA detection test

11

Tx of HPV: methods for removal

remove warts (does NOT eradicate virus so can return)
2. BCA, TCA
3. cryotherapy
4. LEEP
5. podofilox
6. imiquimod
7. intralesional IFN injections

12

BCA: bichloroacetic acid
TCA: trichloroacetic acid

brush on warts: denatures proteins
Tx: HPV
AE: irritated/burned surrounding skin

13

cryotherapy

liquid nitrogen to physically disrupt wart (be careful not to freeze to deep: causes scarring)
Tx: HPV

14

LEEP (loop electrosurgical excision procedure)

removes dysplastic cervical cells
Tx: HPV

15

HSV
1. genome
2. capsid
3. envelope

1. large: dsDNA (has viral DNA pol)
2. icosohedral
3. yes

16

Where does HSV reside in latency?
1. HSV 1
2. HSV 2

peripheral sensory neurons: maintained EXTRACHROMOSOMALLY
1. trigeminal ganglia
2. sacral ganglia

17

HSV replication cycle

1. viral attachement
2. pH-independent plasma membrane fusion
3. release of nucleocapsid that migrates to the nucleus
4. genome is released
5. IMMEDIATE EARLY transcription/translation: makes transcriptional regulators to modify host RNA pol
6. production of EARLY PROTEINS: replicate viral genome: include viral THYMIDINE KINASE and viral DNA POL
7: LATE PROTEINS: capsomeres, envelope, STRUCTURAL proteins
8. virus assembly in NUCLEUS (NUCLEAR INCLUSIONS)
9. virus buds from plasma membrane

18

syncitia

HSV
infected cells fuse with adjacent non-infected cells to form giant cells with more than one nucleus
Mechanism: viral glycoproteins are also present on plasma membrane of infected cells late in infection

19

Dx of HSV

Tzanck smear

20

Tzanck smear

HSV
cells from ulcerous lesion reveal multinucleated giant cells with nuclear inclusion bodies

21

LAT (latency-associated transcript)

HSV
only gene expressed in latency
prevents apoptosis of infected neuron

22

What causes HSV to reactivate?
What effect does this have?

decline in cell mediated immunity
kills infected neuron and recurrent epithelial infection occurs

23

HSV 1 diseases

1. gingivostomatitis
2. herpes labialis (FEVER BLISTER)
3. herpetic whitlow (finger vesicle)
4. KERATITIS
5. conjunctivitis
6. blepharitis
7. ENCEPHALITIS

24

HSV 2 diseases

1. CERVICITIS
2. VULVAR VESICLES
3. vaginal vesicles
4. urethritis
5. PENILE VESICLES
6. perianal vesicles
7. MENINGITIS
8. encephalitis

25

gingivostomatitis

PRIMARY HSV
vesicles on lips, tongue, facial skin around mouth
FEVER, HEADACHE

26

fever blister

RECURRENT HSV
vesicles in some of the same sites as primary after stress
FEVER, HEADACHE

27

How do you determine primary from recurrent HSV genital vesicles?
Sx of both?

difficult; Hx
recurrent: fewer lesions that heal more quickly; more frequent soon after primary infection (diminishes over time)

28

genital HSV Sx

1. vesicles (penis, external/internal vagina; can cause urethritis, cervicitis, vaginitis)
2. flu like
3 itching, buringin in infected area
4. muscle aches of legs, buttocks

29

prodrome symtoms

Sx that can tip HSV sufferer of an impending recurrent infection

30

Can you get HSV if partner doesn't have vesicles?

yes
virus sheds in absence of recurrent vesicles ant after lesions are unapparent