micrococcacea

Question 1

family

Answer 1

micrococcaceae

Question 2

genera

Answer 2

microccocus, staphylococcus, planococcis, stomacoccus (rothia)

Question 3

free living saprophyte

Answer 3

planococcus

Question 4

free living saprophyte, rarely cause human infection

Answer 4

micrococcus

Question 5

normal flora of primates and other mammals

Answer 5

stomatococcus and staphylococcus

Question 6

all, except ______ have been isolated from clinically significant sources

Answer 6

planococcus

Question 7

these genus are always encountered in clinical sources or specimen

Answer 7

micrococcus and staphylococcus

Question 8

can be isolated from clinically significant sources

Answer 8

microccocus
stomatococcus
staphylococcus

Question 9

exists in the environment and doesn’t require or infect a host

Answer 9

planococcus

Question 10

mostly associated with infections

Answer 10

staphylococcus

Question 11

general characteristics (micrococcaceae)

Answer 11

-catalase positive, gram positive cocci
-resident flora of the skin and mucous membranes
-ubiquitous
-infections are directly or indirectly transmitted

Question 12

differential test for micrococcus and staphlyococcus

Answer 12

Lysostaphin susceptibility test
Modified Oxidase test
Bacitracin susceptibility test
oxidation/fermentation (OF) test

Question 13

incubation temp and time for Lysostaphin susceptibility test

Answer 13

35 - 37 deg C, 2 hours

Question 14

antibiotic type of chemical that will destroy glycine rich peptide bond

Answer 14

lysostaphin

Question 15

staphylococcus cannot grow properly in the presence of

Answer 15

lysostaphin

Question 16

reagent use for modified oxidase test

Answer 16

Modified oxidase reagent:
6% tetramethyphenylenediamine hydrochloride in dimethyl sulfoxide
or
Microdase disk ( with impregnated Mod. oxidase reagent)

Question 17

results in modified oxidase test can be observed

Answer 17

within 5-10 seconds

Question 18

positive and negative result for Modified oxidase test

Answer 18

positive: micrococcus ( contains cytochrome oxidase enzyme)
negative: staphylococcus

Question 19

test is used to differentiate micrococcus and stomatococcus from staphylococcus to 0.04 units of bacitracin

Answer 19

Bacitracin susceptibility test

Question 20

_____ of bacitracin inhibits growth of ________ ,while having no effect on ______.

Answer 20

0.04 units, micrococcus/stomatococcus, staphylococcus

Question 21

zone of inhibition should be _____to indicate susceptibility and are typical of : micrococcus

Answer 21

greater than 10mm

Question 22

an organism that typically produce no zone of inhibition in bacitracin susceptibility test

Answer 22

staphylococcus

Question 23

testing if the organism can utilize the carbohydrate on certain environmental conditions

Answer 23

Oxidation/fermentation test

Question 24

uses two tubes of Hugh & leison’s medium

Answer 24

OF test

Question 25

in one tube of OF test the medium is ________

Answer 25

covered with vaseline

Question 26

both tube of OF test has what type of carbohydrate

Answer 26

glucose

Question 27

what dye is used for the ph indicator for OF test

Answer 27

bromthymol blue

Question 28

incubation temp and time duration

Answer 28

35 - 37 deg C and 18-24 hours

Question 29

alternate source of energy for asaccharolytic or non-fermentative organisms

Answer 29

peptone

Question 30

if the medium didnt turned yellow in a close system, what does it indicate

Answer 30

the organism can only utilize the carbohydrate in the presence of oxygen

Question 31

type of organisms that utilizes the carbohydrate in the presence of oxygen

Answer 31

oxidizers

Question 32

if the organism is capable of utilizing the carbohydrate with and without oxygen even in close tube

Answer 32

fermenter

Question 33

with or without oxgyen and no carbohydrate was utilize

Answer 33

nonutilizer

Question 34

will produce acid only in the open tube (without vaseline)

Answer 34

oxidizer (micrococcus)

Question 35

it will produce acid in both tubes

Answer 35

fermenter (staphylococcus)

Question 36

result if Micrococcus was OF tested, what color does it indicate and interpret

Answer 36

open: yellow close: green interpretation: oxidative; strictly aerobic

Question 37

result if Staphylococcus was OF tested, what color does it indicate and interpret

Answer 37

open: yellow close: yellow Interpretation: oxidative and fermentative; facultative anaerobic

Question 38

result of lysostaphin susceptibility test (200ug/ml)

Answer 38

susceptible: staphylococcus resistant: micrococcus

Question 39

result of bacitracin susceptibility test

Answer 39

susceptible: micrococcus resistant: staphylococcus

Question 40

result in furazolidone (100ug)

Answer 40

susceptible: staphylococcus resistant: micrococcus

Question 41

result in lysosome (50-mg)

Answer 41

susceptibility: micrococcus resistant: staphylococcus

Question 42

characteristic of Staphylococci

Answer 42

gram-positive cocci in clusters catalase-positive

Question 43

morphology of staphylococci

Answer 43

spherical cell (0.5-1.5mm) that appear singly, in pairs, and clusters( depends on the plane of division of the organism)

Question 44

most common cause of food poisoning

Answer 44

S. aureus

Question 45

principal virulence factor of S. aureus

Answer 45

coagulated/staphylocoagulase

Question 46

can be cultivated by adding _____ to _____

Answer 46

7.5% to 10% NaCI

Question 47

Some strains produce golden yellow colonies due to the pigment they produce known as ___________

Answer 47

Staphyloxanthin

Question 48

Most colonies beta-hemolytic (halo around the colonyent are presnt

Answer 48

beta-hemolytic