Microscopy, Staining, And Classification Flashcards Preview

Microbiology Exam One > Microscopy, Staining, And Classification > Flashcards

Flashcards in Microscopy, Staining, And Classification Deck (27):
0

What is the proper way to write binomial nomenclature?

Genus' first letter capitalized and everything italicized (species too)

1

What were the old five basic kingdoms?

Animalia, plantae, fungi, protista, prokaryotae

2

Who proposed the three domains of eukarya, bacteria, and archae by comparing nucleotide sequences of rRNA subunits?

Carl Woese

3

What are the new divisions of the eukarya domain?

Animals, plants, fungi, protozoans, algae

4

What is resolution?

The shortest distance between two points on a specimen that can still be distinguished by the observer as separate entities

5

What is contrast?

Differences in intensity between two objects or between an object and background

6

What is contrast important for?

Determining resolution

7

What process in microbiology is important for increasing contrast?

Staining

8

Using stains and dyes in microbiology, what two concepts are enhanced?

Resolution and contrast

9

What is the name of the purple simple stain?

Crystal violet

10

What is the name of the magenta simple stain?

Safranin

11

What is the name of the blue simple stain?

Methylene blue

12

What is the name of the green simple stain?

Malachite green

13

What is the name of the red simple stain?

Carbol fuchsin

14

What is the name of the purple and magenta differential stains?

Gram stain

15

What is the name of the red and blue differential stain?

Acid-fast stain

16

What is the name of the green and magenta differential stain?

Endospore stain

17

What is the result of a gram stain?

Purple gram + cells and magenta gram - cells

18

What type of bacteria is referred to as "acid-fast bacteria"?

Mycobacteria

19

What are the results of the acid-fast stain?

Red acid-fast cells and blue non-acid-fast cells

20

What would happen if you did a gram stain on mycobacteria?

Nothing would show up

21

Are human cells and tissues acid-fast cells?

Noooooooo

22

What are the results of the endospore stain?

Green endospores and magenta vegetative cells

23

What are dichotomous keys?

A series of paired statements that enable a choice to be made about two things to determine what organism is present

24

What type of disease is necrotizing fasciitis?

Emerging

25

What is another name for necrotizing fasciitis?

Flesh-eating disease

26

What is the most common cause of necrotizing fasciitis?

Streptococcus pyogenes (gram + bacteria)