Mid 1: Lec 3

Question 1

Sanders et al ()

Answer 1

Hh is transported between cells using filopodia

Question 2

Hh filopodia what made of and what direction of Hh travel

Answer 2

Actin, mostly anterograde, some retrograde

Question 3

Hh filopodia coreceptors

Answer 3

bind to SHh containing filopodia from other cells

CDO, BOC

Question 5

Sanders et al fluorescent protein markers

Answer 5

actin markers (UCHD) show filopodia, tubulin markers (TAU/MAPT) only at base of filopodia, myosin marker shows the molecular motor that moves the SHH

Question 6

Sanders et al HH size and location

Answer 6

200 nm, stays in filopodia, extracellular leaflet of the filopodial membrane

Question 7

Sanders et al filopodia stability

Answer 7

Filo with SHH are more stable than filo w/o SHH

Question 8

Sanders et al dual expression system

Answer 8

Gli 3 is marked w/ hybrid enhancer and promotor element. SHH is also marked. Shows that filo of SHH cells interact with filo of shh responding cells that contain BOC microdomains

Question 9

3 types Hh signalling

Answer 9

free diffusion, transcytosis, directed transport of signalling molecues and their receptors via filopodia