mid 2: lec 14

Question 1

Ago-CLIP

Answer 1

Use an antibody against argonaught protein Ago.

Different binding sites presumably have different affinities to
Ago and result in more reads

ranking binding sites by the number of reads allows one to
discover the highest affinity and perhaps most relevant binding
sites.

Question 2

mir 450a ovarian cancer paper

Answer 2

mir 450a is possible tumor suppressor

used ago clip to determine targets of mir450a

tested effects of overexpressing mir450a and found it decreases tumorigenicity and metastasis ability

suppressing effect is likely due to suppression of mitochondria genes required for high ox phosph that tumours use to grow

Question 3

tumorigenicity

Answer 3

ability to grow tumor from cells
derived from culture after their injection in
immunodeficient mice.

Question 4

metastatic potential

Answer 4

ability of tumor cells to
migrate and invade other tissues and to survive in the absence of adhesion to the extracellular matrix during their pathway from one organ to another through the
systemic circulation or peritoneal fluid; a process that can be partially mimicked by cell dishes, which prevent cell adhesion