mid 2: lec 15

Question 1

Functional sequestering of microRNA

Answer 1

cellular or foreign rna can sequester mirna: sponge effect

sponge mrna to mirna ratio bigger than target mrna to mirna ratio

sponge rna has more binding sites than target mrna

Question 2

CircRNAs as sponges for miRNAs

Answer 2

can act as regulatory rnas that sequester mirnas

Question 3

CDR1as general inc binding sites inc what important for

Answer 3

circular rna important for sensorimotor gating and synaptic transmission

has binding sites for mir7 and mir671

Question 4

circ rna brain function paper

Answer 4

cdr1as

determined expression pattern using RNA fluorescence in situ hybridization (FISH) : expressed in neurons not glial cells, mostly excitatory neurons

used crispr cas9 to knock out cdr1as locus

neuronal CDR1as either directly
stabilizes or transports miR-7,
despite having >70 binding sites, it
is not an inhibitor of miR-7 function

miR-671 target levels increase,
suggesting that for this miRNA,
CDR1as, is acting like a sponge.

Question 5

adipose circulating mirnas paper

Answer 5

conditionally knocked out dicer in adipose tissues

revealled that adipose tissues are important source of exosomal mirnas

Question 6

exosomal miRNAs

Answer 6

mirnas in vesicles that can be paracrine or autocrine