Mid 2: Lec 13

Question 1

identifying targets of mi rnas

Answer 1

-use transcriptomics to determine effect of mirna
mrna microarrays
next generation sequencing

-Crosslinking and immunoprecipitation

Question 2

CLIP

Answer 2

Crosslinking and immunoprecipitation for determining mirna targets

targets RISC complex

cuts away part of mrna that is not bound

sequencing is done on the bound part to determine the target

Question 3

mirna 122 and hcv

Answer 3

HCV acts as a sponge to sequester miR-122 in the liver.

MIRNA 122 is a tumor suppressor