Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

MOUSE - MOD 4 - ANIMAL CARE, HEALTH AND MANAGEMENT > MOD 4 - COURSE 1 - PT 1 - MICE > Flashcards

MOD 4 - COURSE 1 - PT 1 - MICE Flashcards

(49 cards)

Start Studying
1
Q

Why are mice used so often as lab animals

A

-abundance of literature published regarding them
- ease of handling
- high fertility rate
- short gestation period - 3 weeks
- short generation times - 12 weeks
- low maintenance labour and cost
- relative ease of genetic manipulation
- acceptable model for diverse human disorders and idseases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

list some characteristics about mice

A
  • noctournal & poor visions
  • sensitive to ultrasonic frq
  • susceptible to audiogneic seiqzures - DBA/2
  • thigmotactic - feels secure when enclosed
  • social animal
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what are some unique morphological features of mice

A
  • adrenal gland - spontaneous proliferation of Type A cell
  • bone - lack harvesian systems
  • kidney - parietal cells of bowmans capsule
  • liver - anisocytosis and anisokaryosis
  • lung - bronchus assocaited lumphoid tissue = not present
  • spinal cord - mototr nerve fibers in ventral and lateral column
  • testes - spermatogonia maturation
  • urinary bladder - lymphoid aggregates in lamina propria
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

whats a potential sign of mice mating

A

mucous plug vagina - 24 hours

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what are 3 types pf mating systems mice

A
  • monogamous
  • trio
  • polugamours
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

hwo to determine sex of mice

A

longer distance in males of anurogenital tract

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

do mice have multiple oestrogen cycles per year

A

yes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q
  • when do they reach sexual maturity mice
A

40-60 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

how long is mices oestrous cycle

A
  • 4-5 days
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

whats the gestation period of mice

A

19-21 days 19 for inbred strains

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

litter size of mice

A

6-12 pups

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

how long is weaning age of mice

A

21 days

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what is post partym oestrus

A

occurs 24 hours after birth in mice - leads to successful mating if male = avialable

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what is the outbread stock of mice

A
  • colony - genetic variation
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

charactersitics of outbread stock mice

A
  • good reproduction
  • maintained through random breeding
  • large litter size
  • each individual = genetically uniques - heterozygous animals
  • gentically and phenotypically variable
  • characteristics of colony = change quickly
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

what are some examples of outbred stock mice

A
  • CF1
  • CD1
  • SWISS WEBSTER
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

whats inbread strain of mice

A
  • colony genetically identical individuals
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

characteritics of inbred mice

A
  • homozygous of virtually all genes
  • genetic and phenotypic uniformity
  • long term stability - only hcange if mutation
  • homozygosity - some deletirous recessive gene s- become fixed within each strain - leads to inbreeding depression - seen as poor breeding performance
  • individualality - each isogenic strain - has its own specific subset of characterisitics
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

what are some examples o finbred mice strains

A
  • C57BL/6, DBA, BALB/c
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

whats the microenviornment of lab mice

Study These Flashcards
A
  • primary housing - cage
21
Q

whats the lab mice macroenvironment

Study These Flashcards
A
  • sencondary enclosure - room level
22
Q

what is the light intesnsity and cycle needed for lab mice?

Study These Flashcards
A
  • 14h/10h or 12h/12h light/dark cycles
  • light shouldnt exceed 300lux
  • 60lux for albino mice
23
Q

what temperature & humidity is used for mice

Study These Flashcards
A

20-24’C
40-60% humidity

24
Q

what ventilation rate = reccomened for rodent facilities

Study These Flashcards
A

15-20 Air changes per hour

25
what diet and water should be provided for mice
- specially formulated for rodents - breeding colonies - should be given higher fat diet (6-11%) - protein contect (14% to 22-25%) - water and diet =ad libitum
26
how can odour effect mice and how can you stop it
- strong scents = induce stress reduce breeding - change gloves - between cages
27
what environmental enrichment should be provided for mice
- provided at all times - essentialy that breeding colonies = provided with nesting materials - mice = social = shouldnt be individually housed
28
how is providing correct handling and husbandry for mice important and how should you do this
- keep handling to a min - is breeding pair = stable dont seperate - do not remvoe right before female birth - do not put back in before pups = weaned - avoid spreading scent - maximise darkness - ensure dark cycle = maintained - provide nesting materials - give higher fat or protein content
29
in which annex of the 2010/62/EU directive does it speiciy mandatory minimum standard and accomodations for mice, and where is there furhter guidance
annex VIII - following appendixx A - council of europe conventions ETS 123 - contain supplementary guidance on accomodation, care welfare, enrichment etc
30
how can light that is the wrong intensitity cause damage to mice
may cause retinal degeneration in albino mice especially
31
how does the wrong light cycle negatively impact mice
- variations in light cycle - result in reduced breeding
32
how can noise negatively effect mice
susceptible to audiogenic seizures stress caused by large noises - cause large adrenal gland - reduced breeding efficicency and behvaioural disorders
33
what can low humidity do to mice and rats
- (<35%) - cause ringtail
34
what can poor ventilation do to mice and rats
- high ammonia levels - cause respiratory and immunological alterations
35
what other factors effect mice physiological behaviour
- cage size, cage structure, enrichment materials, NH3 CO2 levels, light intensity and cycle, light wavelenght, noice, odours, human presence
36
why is environemntal stressors so important for science
- effects normal physiologically of animal - so may not right scientific conclusion reuslt can be repeated as there is more variablitiy compared to what you wanted to be the variable
37
what is the circadian rhythm in mice
- 24 hour intervals - enogenously generates - still suscpetible by environment
38
what bahviours of mice are exhibited in circadian rhythm
- locomotor activity - feeding - secretions - sensory processing - learning capabilities
39
what physiology processes of mice = on a circadian rhythm
- control of body temp - cardiovascular function - melatonin secretion - cortisol secretion - metabolism -sleep
40
where is the master circadian pacemaker located in mammals?
- in suprachiasmatic nucleus in rostroventral hypothalamus section of brain (above optic chiasm)w
41
what are the 2 main subdivisions of the supreachiasmatic nucleus
- ventrolateral - or core - region - dorsomedial - or shell - region
42
are neurones in the ventrolaterial region intrinsically rythmic
no
43
what main neurotransmitters do the nerons in teh ventrolaterial region use
GABA (gamma- aminobutiric acid) and VIP (vasoactive intestinal polypeptice)
44
are cells in the dorsomedia region intrinsically rhythmic?
yes
45
what are the main neurotransmitters of the cells in the dosomedia region
- GABA and AVP - ARGININE VASOPRESSIN
46
What affects circadian periods
- environemntal fluctuations - base value = genetically determined - different species = different periods
47
whats Achoff's rule
-species that exhibit free running rhythms in constant light - the period of rhythm = affected by intesnity of light
48
how does changes in photoperiods affect mice
- locomotor activity - behvaiour - different hormone biochemical paramters, stress rersponse - gene expression - oestrus cycle - interupption = effects reproduction
49
breeding mice be mainted on what light cycle
12/12 or 14/20 light/dark cycle

MOUSE - MOD 4 - ANIMAL CARE, HEALTH AND MANAGEMENT (8 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions