MOD 4 - COURSE 1 - PT 3 - MOUSE

Question 1

In what situation do animals not need to be individually mmakred

Answer 1

• instead use small group identification - enough = animals = treated the same manner - no differences in genotype or phenotypes and no individual variation responses to treatment needed to be measured

Question 2

what info is on the cage card

Answer 2

• different colours - can be used for different strains, date procedure started etc
• complete and correct nomenclature
• number and sex of animals
• name of resposible investigator and protocol number for research project
  mouse colonies - indivate pedigree number and reproductive performance history

Question 3

why id identification so important

Answer 3

• loss of identification means the animal = unusable

Question 4

what types of methods are there for individual identification

Answer 4

• temporary methods
• permanent methods

Question 5

what are some examples of non-invasive temporary identification methods

Answer 5

• fur marking
• skin marking/ coat dying

Question 6

what are some examples of temperary invasive identification methods

Answer 6

• subcutaneous injection of ink
• ear tag

Question 7

what are some examples of permanant identification methods that generate tissue samples

Answer 7

• ear notching
• distal phalanx remove

Question 8

what are some examples of permanent identification methods that do not generate tissue

Answer 8

• tattoo methods
• microchip transponders (RFID)

Question 9

what research models using GEM mice

Answer 9

• medical resarch - study functions of specific genes
• molecular biology - analysis of gene expression and how is it regulated
• cancer studies - genes involved in development of cancer
• biotechnology - animals - sources of specific proteins i.e. insuline an dgrowth hormones
• xenotranslantation - development of animals to be used as organ donors

Question 10

what is a health concernof GEM mice

Answer 10

• phenotype is often hard to predict - so could have craxy health propblems

Question 11

what are the potential hazard of working with lab animals to human helath

Answer 11

• lab animal allergy
• injury
• infections
• zoonoses

Question 12

wheres the strongest allergen from animals

Answer 12

• found in urine - mouse urinary proteins

Question 13

what is the best way to prevent lab alnimal allgergy

Answer 13

• reducing the allergen exposures
• gloves and face masks
• handling in laminar flow hoods
• housing in mice in individually ventilated cages

Question 14

what injuries are most common

Answer 14

• physcial hazards
• heavy equipment,
• corrosive chemicals
• bites
• scratches
• handling sharps

Question 15

how can you reduce risks of injuries physcial

Answer 15

• risk assessment and propertraining
• hazard assessment - knowledge and training - PPE needed

Question 16

what protection can be used for protection against infectious agents

Answer 16

• lab coats, gloves, maks
• hand washing
  eating and smoking = prohibited
• remove PPE befroe leaving animal handling

Question 17

what are zoonotic diseases?

Answer 17

• aniaml disease - describe process whereby infectious disease = transmitted between species - from animals othe than human to human or human to aniamls

Question 18

how do zoonotic disease enter the body

Answer 18

• enter body through a variety of methods - broken skin, eyes, mucous membrnae, lungs

Question 19

what is quaritine?

Answer 19

seperation of newly recieved aniamsl - from already in facilities - effective quaritine - minimize introduction of disease agents in established colonies - quarantines period - sufficients to allow ezpression fo diseases oresent in incubation stages