MOD 4 - COURSE 4 - PT 2 HAMSTER Procedures Flashcards
(18 cards)
what are some methods of euthanasia for hamsters othe than fetal larval and embryonic forms
- overdose of anaesthetic using route of anesthetic agent appropriate size and species of hamsters
- exposure to CO2 - rising concentration - up to 1.5kg - NOT NEONATAL HAMSTERS
- dislocation of neck - with prior use to sedative or anaesthesia in rodents - 150g-500g
concussion of brain - stricking the cranium - up to 1kg
what are some methods of fetal, larval and embryonic forms of hamsters
- overdose of anaesthetic drug suitable for stage of development
- decapitation up to 50g
what methods of confirmed killing must be used after schedule 1 killing
- confirmation of permenant cessation of circulation - severing major blood vessel
- destruction of brain
- dislocation of neck
- exsanguination
- confirming the onset of rigour mortis
- instantaneous destruction of body in maceration
what are som e cample of minimally invasive procedures of injection and dosing techniques
- subcutaneous
- intramusclar
- intraperitoneal
- gastric intubation
what is a subcutaneous injection
- fluid injected into spae between skin nad underlying muscle
- absorbed by adjacent vessels in blood stream
what volume can be injected using the subcutaneous method
- 5ml/kg
- needle 21-23 gauge
how do you adminsiter a subcutaneous injection
- restraint by scruff
make a tent of skin at scruff - insert needle at bottom of skin tent
- DO NOT push needle through opposite side of skin tent or into muscle mass
- aspirate - replace fluid if colour = aspirated inject material
whats an intramusclar injection and how do you do this
- caudal thigh muscle - most frequency used for IM injections - care taken to avoid sciatic nerve - runs laterally along hip and hind limb
whats an intraperitoneal injection and how do you it
- injected into peritoneal cavity - absdominal
expose ventral side of hamsters - title head sslightly down and inject lower left quadrant of animals abdomen - avoid organs - apirate syringe before injection - doscard colour fluid do not inject
when do you use intraperitoneal injection
used effectily in neonates - and rodents
whats the volume for IP injections
10ml/kg
what needle gauge go you use for IP injections
- 21-25 gauge
what is a oral gavage
- direct placement - liquid into stomach
how do you perform a oral gavage
- flexible or rigid gavage needle - consider refinement - attached to hypodermic syringes - straight or curved eith ball at end to prevent injruty ( 16-18 gauge neeedle) - 2.5-3inch needle lenght most common
-ENSURE ENTRY NOT IN TRACHEA - restrain- by scruff og neck - measure nedel form corner of mouth to last rib - place tip of gave needle into mouse - slide back down past tounge - should slide down EASILY - DO NOT FORCE - DO NOT ASPIRATE
- administer needle ar moderate rate
- if meeting resistant - then withdraw needle and begin again
how do you do blood collection in hamsters
- not tail vein like mice and rats
- orbital sinus bleed = AVOIDED
- refined method fom lateral saphenous vein
- relatively quick - does not requires hamster to be warmed prior to sample collection
- blood - collected from vein - runs dorsally adn then laterallyy over tarsal joint
- restain hamsters - manually or tube
- not normally require anaesthesia - could be used for welfare if hamster = wriggly
- immobilize in extended position - apply gentle pressure downwards above knee joint
- hair = removed and area = cleaned
- sterile needle puncutre = performed
- blood collected by capillary action in hameacrit tube
- apply gentle pressure over puncture site
what might be observed after blood taken in hamsters
- temporary favouring of opposite leg
how many volume can be takne using this blood method
- 0.15ml for single smaples =- repeated 2 weekly intervals
- multiple smaller smaple - 0.01ml daily
what are some possible adverse affects
- bruising
- haemorrhage
- infections
- temp favouring of opposite limb
