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Non Fermantative Gram Negative Bacilli Flashcards

0
Q

Positive open tube; negative close tube. Non fermenter = Pseudomonas, Alcaligenes

A

Oxidizer or oxidative

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1
Q

Positive both open and close tubes. Fermenters = coliform organisms. E.coli

A

Fermentative (yellow color)

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2
Q

Can only use CHO only in the presence of air

A

Acinetobacter, stenotrophomonas

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3
Q

Both negative in open and close tubes

A

Non oxider

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4
Q

Unable to utilize CHO in the absence or presence of air

A

Non-saccharolytic

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5
Q

Sealed remains green, if unsealed becomes yellow

A

Oxidative fermentation

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6
Q

Slight yellowish on top of both tubes

A

Oxidative or slow fermentation

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7
Q

Widely R to most antibiotics; obligate aerobe, straight gram -, NS, catalase and oxidase +, metabolism is respiratory

A

Pseudomonas

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8
Q

Growth at 35C

A

+ Pseudomonas aeruginosa

- Pseudomonas flavescens

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9
Q

Cetrimide test at 35C for 7 days

A

+ Pseudomonas aeruginosa

- Escherichia Coli

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10
Q

Litmus milk test at 35c for 7 days

A

Acid - pink (E. Faecium) - coagulate casein

Alkaline - blue dot ( A. Faecaelis)

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11
Q

RNA morphology group of pseudomonas: RNA group 1

A

The fluorescens group: P. Aeruginosa, P. Fluorescens and P. Putida
The Stutzeri group: P. Stutzeri and P. Mendocina
The Alcaligenes group: P. Alcaligenes and P. Pseudoalcaligenes

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12
Q

RNA morphology group of pseudomonas: RNA group 2

A

The Pseudomallei group: P. Mallei, P. Pseudomallei and P. Cepacia

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13
Q

RNA morphology group of pseudomonas: RNA group 3

A

The Acidovorans group: P.acidovorans and P. Testosteroni

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14
Q

RNA morphology group of pseudomonas: RNA group 4

A

The Diminuta Group: P. Diminuta and P. Vesiculare

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15
Q

RNA morphology group of pseudomonas: RNA group 5

A

Xanthomonas ( Pseudomonas) maltophila

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16
Q

Used to detect organism type of action of CHO; commonly used by Pseudomonas; oxidize sugars in low levels, oxidase +, can grow in any lactose medium, motile. This test is designed to differentiate bacteria on the basis of fermentative or oxidative metabolism of CHO

A

Oxidative Fermentative Medium

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17
Q

Promotes anaerobic group due to true fermentation, incubate then add mineral oil.

A

Closed OF Medium

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18
Q

No mineral oil is added to allow aerobic growth and oxidation, changes are due to oxidative utilization of CHO present. Stab then incubate.

A

Open OF medium

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19
Q

pH indicator used:
Acid - yellow
Alkaline - blue

A

Bromythmol blue

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20
Q

MAC on Pseudomonas

A

NLF

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21
Q

Important member of Pseudomonas; Also known as Agent of blue pus and previously known as Bacillus pyocyaneus.

A

Pseudomonas aeruginosa

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22
Q

Morphology of Pseudomonas Aeruginosa

A

NS; NE; Motile (monotrichous) except B.mallei; aerobic with pili

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23
Q

Oppurtunistic pathogen of P.aeruginosa

A

Utilizes glucose oxidatively, oxidase + except S.maltophila, growth at 42C, Cetrimide +, NO3 +, Acetamide +

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24
Causative agent of Pseudomonas Aeruginosa
Swimmer's rash or ear; dermatitis "Jacuzzi or hot tub syndrome" and erythema gangrenosum, contact lens infection
25
Virulence factors of P.aeruginosa
Exotoxin A, endotoxins, slime polysaccharide, elastase and alkaline protease, alginate pilli.
26
Cultural characteristics of P.aeruginosa on BAP
Large flat colonies with ground glass appearance and a zone of hemolysis which is a beta hemolysis, bluish green pigment
27
2 distinct hemolysin of P. Aeruginosa
Heat labile phospholipase a | Heat stable glycolipid
28
Turquoise blue phenazine pigment which acts as antibiotic inhibiting growth of other org. Soluble in water, chloroform.
Pyocyanin
29
Water soluble; pyoverdin produces a yellowish green tinge
Flourescein
30
Red
Pyorubin
31
Brown
Pyomelanin
32
Bluish green.
Pyochelin
33
Green
Pyoverdin
34
Colonies of P.aeruginosa tends to give off what?
Musty odor
35
Pathogenecity of P. Aeruginosa
Lipopolysaccharide, endotoxins, Exotoxin A and S. Phosphorylase Chemolysin, cytotoxin- leukocidin
36
Gram stain of P. Aeruginosa
Straight and slender rods
37
BAP, CAP, MAC and Seller's medium in P.aerugionosa
Mucoid colonies; good growth at 42C.
38
Fluorescein but not a pyocyanin; no growth at 42C; causes UTI, Bacteria and wound infection
Pseudomonas fluorescens
39
Brown (buff colored) wrinkled colonies, NLF, 6.5% NaCl+
Pseudomonas Stutzeri
40
K/K with H2S, NLF, oxidase+
Shewanella putrefaeciens
41
Differentiating test that will seperate Burkholderia + and Stenotrophomonas -.
Oxidase test
42
Generally non pathogenic, involved in human with heavy contaminated medical devices. Aerobic, oxidase +, catalase +, NS, motile except B.mallei
Burkholderia
43
Species of Burkholderia
B.mallei, B.galliodis, B.pseudomallei and B.cepacia
44
#2 cause of cystic fibrosis. Causes foot rot to man, septicemia and pneumonia. Survives well in hospital environment, soil, plants, water and disinfectant. Earthy or dirty like odor. Oxidase and LDC+, motile and drug resistant. Pigment is yellow to yellow green. Previously know as P.kingii and P.multivorodans
Burkholderia cepacia
45
Former known as P.mallei and common name is Whitmore's bacillus. Cocoid-rod shaped, oxidase -, unable to grow at 42C, NM.
Burkholderia mallei
46
Occasionally transmitted to man, horse's disease
Glanders
47
Formerly know as P.pseudomallei. Causes melliodis, septicemial glanders like disease, said to emit the earthy odors. Growth at 42C, M. Known as Vietnamese Time bomb
Burkholderia pseudomallei
48
Media used in B.pseudomallei, which gives a dry, wrinkled, violet colonies
Ashdown culture medium
49
Formerly known as Xanthophomonas mallei, not part of the human flora, maltose loving bacteria, Motile, utilizes glucose and maltose aerobically only. Grows on 42c, DNase - and OF +/-, colonized the burnt skin
Stenotrophomonas mallei
50
BAP on Stenotrophomonas mallei
Lavender- green to light purple pigment with ammonia cal smell
51
Motile, oxidase +, non fermantative , NLF, isolated in water and soil including hospital environment, acquired thru contaminated medical device and solution, feather edged colonies.
Alcaligenes faecalis
52
BAP on Alcaligenes faecalis
Surrounded by a zone of green discoloration, alpha-hemolytic. Smell resembles apple or strawberries
53
Member of the neisseriaceae family. Oxidase -, catalase+, NM, Mac-, grm-plump diplococci, + glucose medium, strict aerobe, - in nitrite test, cause UTI, wound and diarrhea. Acquired thru contaminated medical devices
Acinetobacter calcoaceticus
54
Varieties of A.calcoaceticus: acid strains
A.calcoaceticus var anitratum (Herellea vagnicola)
55
Varieties of A.calcoaceticus: non-acid strains
A.calcoaceticus var lwoffi (Mima polymorpha)
56
Glucose oxidizing , non hemolytic (oxidizer)
A.baumaii
57
Difference between A.lwoffi and N.gonorrhea
Is that A.lwoffi can grow on both EMB and Mac
58
Coccobacilli, NLF, can grow on urethra
Oligella
59
Oligella on BAP
Small opaque colonies
60
NM, urea hydrolysis - and oxidase +
Oligella urethralis
61
Motile, urea hyrdolysis and oxidase -
Oligella ureolytica
62
Medium to long straight rods, oxidase, DNase, gelatin hyrdolysis and INDOLE +; NM
Chrysebacterium
63
Before it was know as what? That may be transmitted from birth canal to neonate, may enscapulated, may cause UTI endocarditis and bacteremia
Chryseobacterium (Flavobacterium) meningosepticum
64
Chryseobacterium (Flavobacterium) meningosepticum on BAP and CAP
Yellowish pigment
65
New name of Chryseobacterium
Elizabethkingia meningosepticum
66
Common name is Morax-Axenfeld; agent of blephanoconjunctivitis/angular conjunctivitis. Coccobacilli to medium rod, catalase +z
Moraxnella lacunata
67
Moraxalle lacunata on MAC
No growth
68
Normal flora of the Moraxella lacunata that inhibits the mucous membranes
Nose, throat, other parts of the URT and conjunctiva
69
Oxidase +, catalas +, NM; grm-coccobacilli , asaccharolytic
Moraxella osloensis
70
Differentiation test of Moraxella osloensis - and Neisseria gonorrhea +
CTA ( Cystine Tripticase Agar)
71
Oxidase +
Alcaligenes, flavobacterium, Moraxella, kingella, eikenella
72
Oxidase -
Acinetobacter
73
Catalase +
Acinetobacter, Alcaligenes, Flavobacterium, Moraxella
74
Catalase -
Kingella, eikenella

Microbiology (7 decks)

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