Non Fermantative Gram Negative Bacilli Flashcards Preview

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Flashcards in Non Fermantative Gram Negative Bacilli Deck (75):
0

Positive both open and close tubes. Fermenters = coliform organisms. E.coli

Fermentative (yellow color)

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Positive open tube; negative close tube. Non fermenter = Pseudomonas, Alcaligenes

Oxidizer or oxidative

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Can only use CHO only in the presence of air

Acinetobacter, stenotrophomonas

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Both negative in open and close tubes

Non oxider

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Unable to utilize CHO in the absence or presence of air

Non-saccharolytic

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Sealed remains green, if unsealed becomes yellow

Oxidative fermentation

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Slight yellowish on top of both tubes

Oxidative or slow fermentation

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Widely R to most antibiotics; obligate aerobe, straight gram -, NS, catalase and oxidase +, metabolism is respiratory

Pseudomonas

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Growth at 35C

+ Pseudomonas aeruginosa
- Pseudomonas flavescens

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Cetrimide test at 35C for 7 days

+ Pseudomonas aeruginosa
- Escherichia Coli

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Litmus milk test at 35c for 7 days

Acid - pink (E. Faecium) - coagulate casein
Alkaline - blue dot ( A. Faecaelis)

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RNA morphology group of pseudomonas: RNA group 1

The fluorescens group: P. Aeruginosa, P. Fluorescens and P. Putida
The Stutzeri group: P. Stutzeri and P. Mendocina
The Alcaligenes group: P. Alcaligenes and P. Pseudoalcaligenes

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RNA morphology group of pseudomonas: RNA group 2

The Pseudomallei group: P. Mallei, P. Pseudomallei and P. Cepacia

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RNA morphology group of pseudomonas: RNA group 3

The Acidovorans group: P.acidovorans and P. Testosteroni

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RNA morphology group of pseudomonas: RNA group 4

The Diminuta Group: P. Diminuta and P. Vesiculare

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RNA morphology group of pseudomonas: RNA group 5

Xanthomonas ( Pseudomonas) maltophila

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Used to detect organism type of action of CHO; commonly used by Pseudomonas; oxidize sugars in low levels, oxidase +, can grow in any lactose medium, motile. This test is designed to differentiate bacteria on the basis of fermentative or oxidative metabolism of CHO

Oxidative Fermentative Medium

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Promotes anaerobic group due to true fermentation, incubate then add mineral oil.

Closed OF Medium

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No mineral oil is added to allow aerobic growth and oxidation, changes are due to oxidative utilization of CHO present. Stab then incubate.

Open OF medium

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pH indicator used:
Acid - yellow
Alkaline - blue

Bromythmol blue

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MAC on Pseudomonas

NLF

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Important member of Pseudomonas; Also known as Agent of blue pus and previously known as Bacillus pyocyaneus.

Pseudomonas aeruginosa

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Morphology of Pseudomonas Aeruginosa

NS; NE; Motile (monotrichous) except B.mallei; aerobic with pili

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Oppurtunistic pathogen of P.aeruginosa

Utilizes glucose oxidatively, oxidase + except S.maltophila, growth at 42C, Cetrimide +, NO3 +, Acetamide +

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Causative agent of Pseudomonas Aeruginosa

Swimmer's rash or ear; dermatitis "Jacuzzi or hot tub syndrome" and erythema gangrenosum, contact lens infection

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Virulence factors of P.aeruginosa

Exotoxin A, endotoxins, slime polysaccharide, elastase and alkaline protease, alginate pilli.

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Cultural characteristics of P.aeruginosa on BAP

Large flat colonies with ground glass appearance and a zone of hemolysis which is a beta hemolysis, bluish green pigment

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2 distinct hemolysin of P. Aeruginosa

Heat labile phospholipase a
Heat stable glycolipid

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Turquoise blue phenazine pigment which acts as antibiotic inhibiting growth of other org. Soluble in water, chloroform.

Pyocyanin

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Water soluble; pyoverdin produces a yellowish green tinge

Flourescein

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Red

Pyorubin

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Brown

Pyomelanin

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Bluish green.

Pyochelin

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Green

Pyoverdin

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Colonies of P.aeruginosa tends to give off what?

Musty odor

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Pathogenecity of P. Aeruginosa

Lipopolysaccharide, endotoxins, Exotoxin A and S. Phosphorylase Chemolysin, cytotoxin- leukocidin

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Gram stain of P. Aeruginosa

Straight and slender rods

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BAP, CAP, MAC and Seller's medium in P.aerugionosa

Mucoid colonies; good growth at 42C.

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Fluorescein but not a pyocyanin; no growth at 42C; causes UTI, Bacteria and wound infection

Pseudomonas fluorescens

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Brown (buff colored) wrinkled colonies, NLF, 6.5% NaCl+

Pseudomonas Stutzeri

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K/K with H2S, NLF, oxidase+

Shewanella putrefaeciens

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Differentiating test that will seperate Burkholderia + and Stenotrophomonas -.

Oxidase test

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Generally non pathogenic, involved in human with heavy contaminated medical devices. Aerobic, oxidase +, catalase +, NS, motile except B.mallei

Burkholderia

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Species of Burkholderia

B.mallei, B.galliodis, B.pseudomallei and B.cepacia

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#2 cause of cystic fibrosis. Causes foot rot to man, septicemia and pneumonia. Survives well in hospital environment, soil, plants, water and disinfectant. Earthy or dirty like odor. Oxidase and LDC+, motile and drug resistant. Pigment is yellow to yellow green. Previously know as P.kingii and P.multivorodans

Burkholderia cepacia

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Former known as P.mallei and common name is Whitmore's bacillus. Cocoid-rod shaped, oxidase -, unable to grow at 42C, NM.

Burkholderia mallei

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Occasionally transmitted to man, horse's disease

Glanders

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Formerly know as P.pseudomallei. Causes melliodis, septicemial glanders like disease, said to emit the earthy odors. Growth at 42C, M. Known as Vietnamese Time bomb

Burkholderia pseudomallei

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Media used in B.pseudomallei, which gives a dry, wrinkled, violet colonies

Ashdown culture medium

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Formerly known as Xanthophomonas mallei, not part of the human flora, maltose loving bacteria, Motile, utilizes glucose and maltose aerobically only. Grows on 42c, DNase - and OF +/-, colonized the burnt skin

Stenotrophomonas mallei

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BAP on Stenotrophomonas mallei

Lavender- green to light purple pigment with ammonia cal smell

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Motile, oxidase +, non fermantative , NLF, isolated in water and soil including hospital environment, acquired thru contaminated medical device and solution, feather edged colonies.

Alcaligenes faecalis

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BAP on Alcaligenes faecalis

Surrounded by a zone of green discoloration, alpha-hemolytic. Smell resembles apple or strawberries

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Member of the neisseriaceae family. Oxidase -, catalase+, NM, Mac-, grm-plump diplococci, + glucose medium, strict aerobe, - in nitrite test, cause UTI, wound and diarrhea. Acquired thru contaminated medical devices

Acinetobacter calcoaceticus

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Varieties of A.calcoaceticus: acid strains

A.calcoaceticus var anitratum (Herellea vagnicola)

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Varieties of A.calcoaceticus: non-acid strains

A.calcoaceticus var lwoffi (Mima polymorpha)

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Glucose oxidizing , non hemolytic (oxidizer)

A.baumaii

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Difference between A.lwoffi and N.gonorrhea

Is that A.lwoffi can grow on both EMB and Mac

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Coccobacilli, NLF, can grow on urethra

Oligella

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Oligella on BAP

Small opaque colonies

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NM, urea hydrolysis - and oxidase +

Oligella urethralis

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Motile, urea hyrdolysis and oxidase -

Oligella ureolytica

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Medium to long straight rods, oxidase, DNase, gelatin hyrdolysis and INDOLE +; NM

Chrysebacterium

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Before it was know as what? That may be transmitted from birth canal to neonate, may enscapulated, may cause UTI endocarditis and bacteremia

Chryseobacterium (Flavobacterium) meningosepticum

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Chryseobacterium (Flavobacterium) meningosepticum on BAP and CAP

Yellowish pigment

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New name of Chryseobacterium

Elizabethkingia meningosepticum

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Common name is Morax-Axenfeld; agent of blephanoconjunctivitis/angular conjunctivitis. Coccobacilli to medium rod, catalase +z

Moraxnella lacunata

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Moraxalle lacunata on MAC

No growth

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Normal flora of the Moraxella lacunata that inhibits the mucous membranes

Nose, throat, other parts of the URT and conjunctiva

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Oxidase +, catalas +, NM; grm-coccobacilli , asaccharolytic

Moraxella osloensis

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Differentiation test of Moraxella osloensis - and Neisseria gonorrhea +

CTA ( Cystine Tripticase Agar)

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Oxidase +

Alcaligenes, flavobacterium, Moraxella, kingella, eikenella

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Oxidase -

Acinetobacter

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Catalase +

Acinetobacter, Alcaligenes, Flavobacterium, Moraxella

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Catalase -

Kingella, eikenella