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Biochemical Tests Flashcards

0
Q

Contains lactose, glucose, sucrose and iron and forms H2S

A

Triple Sugar Iron

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1
Q

Utilization of carbohydrates by bacteria. In bacteriology, observing color changes in pH indicator as acid products are formed.

A

Fermentation

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2
Q

If glucose are fermented, butt will produce and slant will produce what?

A

Butt becomes yellowish and Slant becomes initially yellow.

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3
Q

Since glucose is in low conc., organism in aerobic conditions uses Peptone medium, slabs will become what?

A

Slant becomes alkaline

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4
Q

TSI organism that ferments lactose will have an?

A

A/A reaction

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5
Q

A/A with gas on TSI

A

E.coli

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6
Q

A/A with H2S on TSI

A

Citrobacter freundii

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7
Q

K/A with gas and H2S on TSI

A

Proteus mirabilis

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8
Q

K/K on TSI

A

Pseudomonas aeruginosa

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9
Q

TSI

A

pH indicator ( phenol red)

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10
Q

Result on CHO Fermentation Test: NLF

A

Edwardsiella, Morganella, Hafnia, Proteus, Providencia, Salmonella, Yersinia, Shigella, Serratia

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11
Q

Result on CHO Fermentation Test: LF

A

Enterobacter, Escherichia, Klebsiella

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12
Q

Result on CHO Fermentation Test: LLF

A

Citrobacter, Arizona

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13
Q

Two enzymes that must be present in the Klebsiella-Enterobacter-Hafnia-Serratia Group.

A

Beta-galactoside permease

Beta-galatosidase

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14
Q

Many of anaerobic bacteria that produces large quantities of butyric acid

A

Clostridium, Peptostreptococcus, Fusibacterium

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15
Q

Contains lactose, glucose and iron salt

A

Kligler’s Iron Agar

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16
Q

Reactions of KIA on P. Aeruginosa

A

K/K

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17
Q

Reactions of KIA on Shigella sp.

A

K/A

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18
Q

Reactions of KIA on Samonella, Citrobacter, Arizona and some Proteus

A

K/A with gas and H2S

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19
Q

Reaction of E.coli and Klebsiella-Enterobacter group

A

A/A

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20
Q

Indicators: sodium thiosulfate and ferrous sulfate

+ black color or black ppt

A

H2S production

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21
Q

+ bubble formation or splitting of the media or complete displacement of the media from the bottom of the tube

A

Gas Production

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22
Q

Glucose and lactose

A

Russell’s Double Agar

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23
Q

Structure similar to lactose that detects the enzyme from beta-galatosidase. Used to distinguish enteric bacteria (Salmonella -, Citrobacter +) and identify Pseudomonas. Similar to lactose, except glucose replaced ton ONPG

A

Beta-galactosidase and ONPG Test Orthonitrophenyl beta-d-galatopyaranoside

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24
Chromophere that is released into the medium and detected by a pale yellow color.
Orthopnitrophenol
25
Result in Beta-galatosidase and ONPG test
+ Yellow | - no color change
26
SIM test: Ability of an organism to split tryptophan to form compound indole. + pink to wine colored ring
INDOLE production
27
SIM: + blackening of agar
Sulfide
28
Use for initial grouping of enterobacteriaceae
IPViC
29
Production from tryptophan
INDOLE
30
Production from phenylalanine
Phenylpyruvic acid
31
Due to the formation of acetoin from pyruvate in glucose broth
Positive Vogues-proskauer test
32
Ability to utilize as a single carbon source
Citrate
33
Acidification of glucose broth due to formation of mixed carboxylic acids from pyruvate
Methyl red test
34
Ability of an organism to produce INDOLE from tryptophan
INDOLE test
35
Media used in INDOLE test
Tryptophan Agar
36
Reagent used in Indole test
Ehrlich's reagent
37
Result of INDOLE test
+ pink to wine colored ring | - no color development
38
Screening for INDOLE production
Rapid spot INDOLE test
39
Reagent used in Rapid spot indole test
P-aminocinnamaldehyde
40
Ability of organism to produce and maintain stable end products from glucose fermentation; mixed acid glucose fermentation
Methyl red test
41
Indicator used in methyl red test
Methyl red
42
Results in Methyl red test
+ distinct red or bright color red ( E.coli) | - yellow or no color change (E.cloacae)
43
Butylene glycol of glucose formation; ability of an organism to produce acetoin
Vogues-Proskauer test
44
Media used in Vogues-Proskauer test
MRVP or Clark Lubs Broth
45
Reagent used in Vogues-Proskauer test
Alpha napthol and KOHn(Barritt's method)
46
Results in Vogues-Proskauer test
+ pink to red color (E.cloacae) | - no color change (E.coli)
47
Other reagent used in vogues-Proskauer test
Apha napthol in 40% KOH creatine (Coblentz method)
48
Result in vogues-Proskauer test
+ red (Strep.mutans) | - yellow (strep mitis)
49
Key biochemical property of Salmonella, Klebsiella, Serratia, Enterobacter and Citrobacter. Ability of an organism to utilize sodium citrate as a carbon source and inorganic ammonium salts as a nitrogen source.
Citrate Utilization test
50
Media used in Citrate utilization test
Simmon Citrate Agar
51
Reagent used in Citrate Utilization test
Bromythmol Blue
52
Results in citrate utilization test
+ intense blue color (Klebsiella, Enterobacter, Pseudomonas) | - green color ( Escherichia and Edwardsiella)
53
Enzyme produced by some aerobic bacteria as part of the respiratory oxidation mechanisms. P.aeruginosa and N.meninggitis
Oxidase test (Cytochrome oxidase/ indophenol blue)
54
Filter paper method of Oxidase test
Tetramethyl p-phenylalanine diamine dihydrocholride
55
Results in Oxidase test
+ bluish purple ( P.aeruginosa) | - E.coli
56
To produce enzyme urease. | Some organism hydrolyze urea rapidly releasing ammonium and CO2.
Urease test
57
Strong urea producer
Providencia, Proteus, Morganella
58
Weak urea production
Klebsiella
59
Urease producer
Yersinia Enterocolitica
60
Results of urease test
+ pink/magenta P.vulgaris and S.marcesens | - no color change
61
Slow urease producer
Citrobacter, Klebsiella, Enterobacter
62
Rapid Urease Production
Proteus and Providencia rettgeri, K.pneumonia, K.oxytoca, E.cloacae and Yersinia enterocilitica
63
Media used in Urease test
Christensen's Urea Agar
64
pH indicator used in Urease test
Phenol red
65
Some organisms can deaminate phenylalanine converting it to phenylpyruvic acid
Phenylalanine Deaminase Test
66
Results of Phenylalanine Deaminase Test
+ dark green color after addition of ferric chloride
67
Media used in Phenylalanine Deaminase test
Phenylalanine Agar or Tryptohpan agar
68
Reagent used in Phenylalanine Deaminase test
10% Ferric Chloride
69
Results in Phenylalananine Deaminase Test or the reagent Alpha napthol in 40% KOH in creatine (Coblentz method)
+ green color in slant (P.vulgaris) | - E.coli
70
Some organisms can Decarboxylase Lysine converting it to cadaverine
Lysine Decarboxylase Test
71
Indicator used in Lysine Decarboxylase Test
Bromcresol blue
72
Medium used in Lysine Decarboxylase Test
Lysine Iron Agar or Moeller's Agar
73
Result in Lysine Decarboxylase Agar
+ remains purple denoting an alkaline medium
74
Used to determine gram - can Decarboxylase or deaminates Lysine and form H2S
Lysine iron agar test
75
Media used on LIA.
Lysine Iron Agar contains Lysine, Glucose, Peptones, Ferric NH4 Citrate and Na thiosulfate
76
If glucose is fermented
Butt becomes acid
77
If Decarboxylase is not produce
Butt remains acid
78
If oxidative deamination of Lysine occurs
It forms burgundy color on slant in the presence of Ferric Ammonium Citrate and Flavin mononucleotide
79
If deamination do not occur
LIA slant remains purple
80
K/K on LIA reaction
- Lysine deamination | + Lysine Decarboxylation
81
K/A on LIA reaction
- Lysine deamination | - Lysine Decarboxylation
82
R/A on LIA reaction
+ Lysine deamination | - Lysine Decarboxylation
83
H2S indicator on LIA reaction
Ferric ammonium citrate
84
Indicator on LIA reaction
Bromcresol purple
85
Test measures the ability of an organism to decarboxylate an amino acid to form an amine.nit requires acid in pH and anaerobic environment.
Decarboxylase test (Moeller's method)
86
Indicator used in Decarboxylate Test
Bromcresol purple
87
Decarboxylation of the amino acids results in
Alkaline pH change
88
3 decarboxylate broth
Lysine, Arginine, Orthinine
89
Result in Decarboxylase test
+ alkaline purple color
90
If an enterobacteriaceae contains amino acid Decarboxylase, amines produced by Decarboxylase action cause an alkaline pH. Lysine, Arginine, Orthinine are utilized. A base broth without amino acid is included in which glucose fermentation acidifies the broth.
Amino Acid Decarboxylase
91
Lysine ➡️❓
Cadaverine (decarboxylase)
92
Orthinine ➡️❓
Putrescine (decarboxylase)
93
Arginine ➡️❓
Citrulline (dihydrolase)
94
Conversion of Arginine to Citrulline
Dihydrolase reaction
95
Decarboxylation of patterns are essential for the genus identification
Klebsiella, Enterobacter, Escherichia and Salmonella
96
Decarboxylation of patterns are essential for the species identification
Enterobacter cloacae, Enterobacter aerogenes, proteus mirabilis, shigella sonnei
97
Systems for H2S production
``` Lead acetate paper SIM tube Hektoen and SS agar XLD agar Triple Sugar-Iron Agar ```
98
H2S producing
Edwardsiella, Salmonella, Citrobacter, Proteus
99
Media used in H2S
TSI, LIA, SIM, HEA
100
Result in H2S production
Production of black color
101
Non motile on Motility test
Shigella and Klebsiella
102
Non motile on 35C and motile on 22-25C on motility test
Yersinia
103
Ability of organism to produce protease that hydrolyzes gelatin and liquify solid gelatin medium. Used in identification of Clostridium, Flavobacterium, Pseudomonas and Serratia.
Gelatin Liquifacation
104
Results in gelatin Liquifacation.
+ gel liquifies (P. Vulgaris) | - gel solidifies (E.aerogenes)
105
Determine the ability of an organism to reduce nitrate to nitrate by adding sulfanilic acid and alpha-napthylamine
Nitrite Reduction
106
Result in Nitrite reduction
+ red, water soluble azo dye (E.coli)
107
MUG test and result
4-methylumberriferyl-beta-D-glucoronide + blue fluorescence (E.coli) - no fluorescence (P.aeruginosa)
108
Result in Esculin Hyrdolysis
+ black (K.pneumonia) | - yellow (S.Flexneri)
109
KCN Broth results
- clear | + turbid
110
Mako ate Utilization Test
- green | + blue
111
String Test
ID of Vibrio
112
Reagent of String test
0.5% sodium desoxycholate
113
Result on String test
+ string like

Microbiology (7 decks)

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